Team:TU Delft/Project/tolerance/characterization



Characterization of Survival Parts

The tolerance of our bacteria to organic solvent and salt was tested by studying its growth under varying conditions. The solvent tolerance was tested under varying concentrations of cyclo-hexane, 10%(v/v) octane-hexane mixture and n-hexane, biomass production was monitored by measuring the absorbance at 595 nm in M9 medium.

On the other hand, the salt tolerance was tested by using NaCl in LB and M9 (containing 40 mM casaminoacids) media from 0 to 1M salt concentrations, it was compared to another of our biobricks which had the same antibiotic resistance, and same expression level (but of another protein).