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Team:Stockholm/7 July 2010
From 2010.igem.org
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Andreas
Designed primers for some of our parts:
- CPPs (5'-phosphorylated primers for ligation)
- LMWP
- >LMWP_N_F1
- p-AATTCGCGGCCGCTTCTAGATGGTTTCTCGTCGTCGTCGTCGTCGTGG
- >LMWP-CN_F2
- p-TGGTCGTCGTCGTCGTACCGGTTAATACTAGTAGCGGCCGCTGCA
- >LMWP_CN_R1
- p-GCGGCCGCTACTAGTATTAACCGGTACGACGACGACGACCACCACGACGA
- >LMWP_N_R2
- p-CGACGACGACGAGAAACCATCTAGAAGCGGCCGCG
- >LMWP_C_F1
- p-AATTCGCGGCCGCTTCTAGATGGCCGGCGTTTCTCGTCGTCGTCGTCGTCGTGG
- >LMWP_C_R2
- p-CGACGACGACGAGAAACGCCGGCCATCTAGAAGCGGCCGCG
- TAT
- >TAT_N_F1
- p-AATTCGCGGCCGCTTCTAGATGTACGGTCGTAAAAAACGTCGTCAGCG
- >TAT_CN_F2
- p-TCGTCGTACCGGTTAATACTAGTAGCGGCCGCTGCA
- >TAT_CN_R1
- p-GCGGCCGCTACTAGTATTAACCGGTACGACGACGCTGACGACG
- >TAT_N_R2
- p-TTTTTTACGACCGTACATCTAGAAGCGGCCGCG
- >TAT_C_F1
- p-AATTCGCGGCCGCTTCTAGATGGCCGGCTACGGTCGTAAAAAACGTCGTCAGCG
- >TAT_C_R2
- p-TTTTTTACGACCGTAGCCGGCCATCTAGAAGCGGCCGCG
- Transportan 10
- >Tra1_N_F1
- p-AATTCGCGGCCGCTTCTAGATGGCGGGTTACCTGCTGGGTAAAATCAAC
- >Tra1_CN_F2
- p-CTGAAAGCGCTGGCGGCGCTGGCGAAAAAAATCCTGACCGGTTAATACTAGTAGCGGCCGCTGCA
- >Tra1_CN_R1
- p-GCGGCCGCTACTAGTATTAACCGGTCAGGATTTTTTTCGCCAGCGCCGCCAG
- >Tra1_N_R2
- p-CGCTTTCAGGTTGATTTTACCCAGCAGGTAACCCGCCATCTAGAAGCGGCCGCG
- >Tra1_C_F1
- p-AATTCGCGGCCGCTTCTAGATGGCCGGCGCGGGTTACCTGCTGGGTAAAATCAAC
- >Tra1_C_R2
- p-CGCTTTCAGGTTGATTTTACCCAGCAGGTAACCCGCGCCGGCCATCTAGAAGCGGCCGCG
- MITF
- >MITF_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCCTGGAAATGCTAGAATATAATCAC
- >MITF_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTACAAGTGTGCTCCGTCTC
- Protein A, Z domain
- >ProteinA_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGTAGACAACAAATTCAACAAAGAACAAC
- >ProteinA_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTTTTCGGCGCCTGAGCATCAT
- Vitamin B9 cluster
- FolP
- >B9_FolP_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCTATGGCATCATGAATATTACGC
- >B9_FolP_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTATCATTATTCGATAACCAATAGCC
- Xtp2
- >B9_Xtp2_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCACGACGACTTGGTTGATTG
- >B9_Xtp2_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTAAGTTTTGTGATTAATTTTAAGGCG
- FolC2
- >B9_FolC2_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGATACAGGGACGATTGAACAACG
- >B9_FolC2_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTTTGGCTTAACCCCGGCC
- FolE
- >B9_FolE_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCATTGATGAGAAGAACCAAGCAAAG
- >B9_FolE_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTTGCGATTCGCTGTAAGAATTC
- FolK
- >B9_FolK_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGCAAGTAGGGAAGAACGGG
- >B9_FolK_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTATCATCTAACTCACTCACATTTCTTAC
- FolB
- >B9_FolB_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGGCATGATTCGAATTAATAATTTACGCTT
- >B9_FolB_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGCCATTCGGCGTCCC
- Tyrosinase
- >Tyrosinase_F
- GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGCGTGGCTGGTCGGCAAG
- >Tyrosinase_R
- GAACTGCAGCGGCCGCTACTAGTATTAACCGGTGGCGGACACTATGGCTATTTCTAG
- bFGF
- >bFGFF
- GAATCTAGATGGCCGGCGCAGCCGGGAGCATCACC
- >bFGFR
- GAAACTAGTATTAACCGGTGCTCTTAGCAGACATTGGAAGAAAAAGT
- pSB1X3 verification primers
- >pSB1_VF2
- tgccacctgacgtctaagaa
- >pSB1_VR
- attaccgcctttgagtgagc
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