"

From 2010.igem.org

Johan

Made new competent cells (as old one didnt work)

100 ml instead of 800 ml -> 3 tubes, 33 ml in each

7) 50 ml CaCl2, 17 ml in each, then 1 tube

10) 16/8 = 2. 2/3 (3 tubes) = 0,67 ml CaCl2

Aliquotes of 500 µl and 100 µl