Team:Queens-Canada/5 July 2010

From 2010.igem.org

July 5, 2010

PCR

Thai and Yuli

pOdr-1, pOdr-10, pSrb-6

Program run using HIFI with an extension time of 125 s and annealing temperature of 57 °C.

Gel Electrophoresis

Thai and Yuli

pOdr-1, pOdr-10, pSrb-6, eYFP

Used 0.35 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.

PCR

Thai and Yuli

pOsm-10, pStr-220

Program run using HIFI with an extension time of 35 s and annealing temperature of 55 °C.