Team:Queens-Canada/5 July 2010
From 2010.igem.org
July 5, 2010
PCR
Thai and Yuli
pOdr-1, pOdr-10, pSrb-6
Program run using HIFI with an extension time of 125 s and annealing temperature of 57 °C.
Gel Electrophoresis
Thai and Yuli
pOdr-1, pOdr-10, pSrb-6, eYFP
Used 0.35 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.
PCR
Thai and Yuli
pOsm-10, pStr-220
Program run using HIFI with an extension time of 35 s and annealing temperature of 55 °C.