Team:Queens-Canada/25 August 2010

Miniprep

Chris and Yuli

pFlp-1, pSip-1, mCherry, pOdr-10, ChR2::eYFP::unc-54 3'UTR, pRab-7, pCeh-12

Note: [1] pFlp-1::1C3 was dropped in the waste beaker (open) before solution I was added - probably contaminated.

Gel Electrophoresis

Chris and Yuli

pFlp-1, pSip-1, mCherry, pOdr-10, ChR2::eYFP::unc-54 3'UTR, pRab-7, pCeh-12

Split into one 40-lane gel and one 28-lane gel. Used 1 g agarose in 140 mL TBE. Gels run for 40 mins at 100 V.

Digestion

Yuli

pFlp-1::1C3, pSip-1::1C3, mCherry::1C3, pOdr-10::1C3, ChR2::eYFP::1C3 N, pRab-7::1C3, pCeh-12::1C3

Cut with NotI.

Liquid Culture

Yuli

pStr-1, pGpd-2, pHsp-3, pSra-10, pOdr-1, pOsm-10, pMec-7, eCFP::unc-54 3'UTR::1A3, pMec-7::eCFP::unc-54 3'UTR::1C3