Team:Queens-Canada/22 June 2010

From 2010.igem.org

June 22, 2010

Digestion

Yuli and Hao

pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP, unc-54 3'UTR

Cut using XbaI and SpeI. Incubated at 37 °C for 1 hour. Enzymes inactivated at 80 ° C for 20 mins.

Ligation

Hao and Yuli

pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP, unc-54 3'UTR

Ligated using T4 ligase.

Gel Electrophoresis

Hao and Thai

unc-54 3'UTR digestion & ligation products