Team:Queens-Canada/20 August 2010

Miniprep

Yuli and Thai

NpHR::eCFP::1C3, unc-54 3'UTR::1C3, pGpd-2::1C3, pCeh-12::1C3, ChR2::eYFP::unc-54 3'UTR::1T3, 3::unc-54 3'UTR::1T3

Gel Electrophoresis

Chris and Yuli

NpHR::eCFP::1C3, unc-54 3'UTR::1C3, pGpd-2::1C3, ChR2::eYFP::unc-54 3'UTR::1T3, pCeh-12::1C3, eCFP::unc-54 3'UTR::1T3

Split into one 28-lane and one 40-lane gel. Used 1.4 g agarose in 140 mL TBE. Gels run for 40 mins at 100 V.

Glycerol Stock

Chris and Yuli

eYFP, pHsp-3, pMec-7, pStr-220, pSra-10

Compostion: 270 µL 80% Glycerol, 480 µL ddH2O, 750 µL cell culture.