From 2010.igem.org
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PCR program: Touch-Down with Phusion
| 98°C
| 1 min
|
| 98°C
| 10 sec
|
| 59°C
| 20 sec
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| 73°C
| 20 sec
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| return to step 2 for 2 cycles
|
| 98°C
| 10 sec
|
| 57°C
| 20 sec
|
| 73°C
| 20 sec
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| return to step 6 for 2 cycles
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| 98°C
| 10 sec
|
| 55°C
| 20 sec
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| 73°C
| 20 sec
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| return to step 10 for 2 cycles
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| 98°C
| 10 sec
|
| 53°C
| 20 sec
|
| 73°C
| 20 sec
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| return to step 14 for 2 cycles
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| 98°C
| 10 sec
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| 51°C
| 20 sec
|
| 73°C
| 20 sec
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| return to step 18 for 2 cycles
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| 98°C
| 10 sec
|
| 49°C
| 20 sec
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| 73°C
| 20 sec
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| return to step 22 for 2 cycles
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| 95°C
| 30 sec
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| 53°C
| 30 sec
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| 72°C
| 30 sec
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| return to step 26 for 29 cycles
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| 73°C
| 10 min
|
| 12°C
| forever
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