Project Abstract

Our project is on Type lll Secretion Apparatus which is one of the most amazing biological devices. It can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. This apparatus which looks like a syringe is an organelle of pathogenic gram-negative bacterium such as Salmonella and Yersinia. We are aiming at making this device available for E. coli. Because it will not involve usage of pathogenic strains, it will be safer to use. Also we will show how to construct proteins for secretion and how to measure if it is really secreted using GFP.

The Experiments