"

From 2010.igem.org

Melting temps set to 55deg salt conc 50mM

Val239-->Ile

CCGGGTACGGCTaTTCTGACTGTTGTCCCTTTTCACCATGG Tm:84.29 dG:0.38

CCATGGTGAAAAGGGACAACAGTCAGAAtAGCCGTACCCGG Tm:84.29 dG:0.48

Asn286-->Ser

GCAAGACTACAAGTGCACTAgTGTGATTCTGGTCCCGACGC Tm:82.98deg dG:0.75kJ/mol

GCGTCGGGACCAGAATCACAcTAGTGCACTTGTAGTCTTGC Tm:82.98deg dG:1.20

Gly326-->Ser

CTCCGTTGAGCAAAGAAGTAaGCGAGGCAGTGGCACGTCGC Tm:87.43deg dG:0.89

GCGACGTGCCACTGCCTCGCtTACTTCTTTGCTCAACGGAG Tm:87.43deg dG:1.16

His433-->Tyr

GCTACTACGATGAAGAGAAAtACTTTTTCATTGTTGATCGC Tm:74.77 dG:1.47

GCGATCAACAATGAAAAAGTaTTTCTCTTCATCGTAGTAGC Tm:74.77 dG:1.43

Pro452-->Ser

CCTGATCAAGTACAAGGGTTACCAAGTCtCGCCAGCGGAGC Tm:84.24 dG:0.04

GCTCCGCTGGCGaGACTTGGTAACCCTTGTACTTGATCAGG Tm:84.24 dG:0.95

mutagenesis primer

tttacggtctttaaaaaggccgtaatatccagctgaacgg Tm: 77.62 dG: 0.28

tacctataaccagaccgttcagctggatattacggccttt Tm: 77.62 dG: 0.42