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From 2010.igem.org

Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10 pg 26

1) we will pick 2 colonies from each plate and transfer 15 mL LB – Lennox

2) and 50 mL LB – Lennox with E – Coli & Agrobacteria growth. ( 2 tubes)

3) poured (2) 40 mL tubes of LB then added 40 mL of Amp to them

4) poured (4) 14 mL tubes of LB then added 14 mL of Amp to them Did all this inside Biological fume hood to keep sterile

Now I will make LB – Lennox Broth (1L)

1) Weigh out 10 g LB – Lennox Broth powder and place into 500 mL 18 ohm H2O and dissolve inside microwave using Beverage setting 8 oz then autoclave

2) Repeat (2) twice for (1L)

3) 1) 10.0266 g 2) 10.0698 g

4) Placed each into 1L Capped Elenmyier flasks and added using a 500 mL grad cylinder filling with 500 mL 18 ohm H2O and adding 500 to each 1L flask

5) Capped and microwaved on beverage setting then shook to mix

6) Then placed in autoclave on liquid setting/ 121 degrees C for 15 min will place into fridge for use.