"

From 2010.igem.org

Preparing SOB

- SOB BROTH(DRY MEDIA) CatNo. S0221 Lot: S022130D0901

Scale: Mad by Adventurer Pro AV64 s/n: 8030391129 Error+ .67% Calibrated by Cassidy

1) I used 4 - 250 mL capped elenmyer flasks

2) in two of them I added 6.97 grams of SOB media powder to 250 mL DI water

3)the mixed and dissolved on the hot plate

4) once dissolved, I separated the 2 - 250 mL SOB media into 4 - 250 mL elenmyer flasks and autocalved for one hour