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From 2010.igem.org

Today I’m testing absorbance of our trials @ 395nm

1) .733 @ 395nm .678 @ 395nm

2) .855 @ 395nm .586 @ 395nm

3) .892 @ 395nm .693 @ 395nm

4) .708 @ 395nm .733 @ 395nm

5) .700 @ 395nm .700 @ 395nm

6) .693 @ 395nm .729 @ 395nm Blank) .001 @ 395nm .001 @ 395nm

1) I will blank the spec using LB/Lennox made by GM on 4/9/10

2) –Change- I blanked using the LB – Lennox Broth w/200 mL of SOB media – both made by me.

3) I took and used the same Cuvette for every spec scan washing with DI water between every trial

4) Placing about 1 mL every trial 5) Set spec to 395nm we believe that their was too much absorbance in trials 4,5, & 6 so we will do same procedure using florimider. Results say that the GFP is not being expressed in the presence of (AHL)/#3)

- Prof T will now take sample ran in spec and run inside florimidor to check UV absorbance blanking with LB/SOB mixture. Now prof. T. is running the samples in the Fluo. he is blanking w/LB-SOB using 490ex and 510 EM filters Blank read out to .009 +/- .0006 10005 Fluo units for T9002 inside E – Coli Keeping Vol. the same for all spls. blank again before running spls. now running

(A) (0) Fluo units we have no florescence

(B) (160) Fluo units a little bit of florescence

(C) (0) Fluo units this means GFP not producing

(D) (0) Fluo units expected

(E) (0) Fluo units expected

(F) (0) Fluo units expected

Results show no presence of GFP so we will break cells open then test again.