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From 2010.igem.org

9/24/10

1-pull sample of 500 ul of A. Tumefaciens , T9002 and A. Tumefaciens only w/ sterile loop and suspend in 1 ml of broth separately (microfuge tubes steril) (LB Broth made by JH 9/17/10)

2- Pull 10ul of agro+T9002 and 500up agrao sub and 500 ul of LB Broth and add it to a steril tube.

- I’m going to do this procedure 2 more times (A-C)

3- Next I’m ging to pull 10 ul of A.Tmefaciens (only) Sample. (the one I pulled from platae and suspended) then I’m going to pull 500 ul of LB Broth and 500 ul of e.coli sup. And add all three to sterile tube.

- Im going to do the same proc 2 more times (D-F)

4- I’m then going to get 3 steril microfuge tubes and ad 1 mil of LB broth (same broth from prev steps) to cach tube. Then I’m going to add 10 ul of e.coli sup (same from prev steps and 10 ul of Agro-T9002 (same sample that I Pulled from plate)

5- Then put all the tubes A-I inot the small 30C dry incubator @ 3:30 pm 9/24

--for dgarveyRchamberlin 21:55, 26 October 2010 (UTC)