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From 2010.igem.org

9/15/10

- Today we will be extracting DNA from out electroporated cells/T9002 - Wash our grown pos. bacteria with 10 percent glycerol to create Electro comp. cells - Then Electroporate T9002 into Elect. comp grown pos/t bacteria

First we will be extracting DNA from BBa_T9002 Electroporated Cells using this protocol.

9/15/10

I poured 10 mL LB – Lennox into 7 different 15 mL sterile Centrifuge tubes “2” for our electroporated parts T9002, K091107 with 10 mL Amp added 5 for our grown t/pos bacteria w/out Amp labeled then added one loop full of bacteria into each then placed on shaker overnight. Irene streaked our bacteria to set up for grown staining then placed into 37 degree incubation overnight.