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From 2010.igem.org

9/14/2010

Today we found growth from our Electroporated E – Coli parts now we took 10 mL made by GM 4/10/10 and poured 10 mL into 3 sterile tubes then added 10 mL Amp to each tube, then placed a large Colony from each part into these tubes then placed on shaker until tomorrow. - We have discovered that yeast will take a bunch of trial and error so we will try and place our IGem into Grown t/pos Bacteria-

- We will be using-

-For now – I took my 10 mL Centrifuge tubes that had electroporated cells containing parts made Friday and placed into 500 mL 40 percent glycerol and 500 mL of each part and made “2” of each to be frozen as backups.