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From 2010.igem.org
- 21:01, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Transformation
- 21:00, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Transformation (New page: <br>MAIN STEPS/TIME TABLE <br>________________________________________ <br>- Fresh o/n bacteria <br>- LB Agar plate preparation[30 min] <br>- Transformation [2.5-3.0 h] <br>- Incubation o...)
- 20:52, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Protein expression (New page: <br>MAIN STEPS/TIME TABLE <br>- Pre-culturing/5 hours <br>- Culturing/5 hours <br>- IPTG induction/15 hours <br>Materials <br>- LB medium with a suitable antibiotic <br>- TB medium with ...)
- 20:48, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Ethanol precipitation
- 20:48, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Ethanol precipitation (New page: <br>MAIN STEPS/TIMETABLE <br>________________________________________ <br>Total time required to complete the procedure is 1h 40min. <br>MATERIALS <br>__________________________________...)
- 20:44, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/SDS-Page (New page: <br>MAIN STEPS/TIME TABLE <br>________________________________________ <br>- Solution preparation [2 hours] <br>- Cleaning of aparatus [20 min] <br>- Seperating gel preparation and powing...) (top)
- 20:32, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Genomic DNA purification (top)
- 20:31, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Genomic DNA purification (New page: <br>CHECK-LIST PROCEDURE <br>________________________________________ <br>- Harvest up to 2x10^9 bacterial cells in a 1.5 or 2 mL microcentrifuge tube by centrifugation for 10 min at 500...)
- 20:26, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Restriction Digestion (New page: <br>MAIN STEPS / TIMETABLE <br>________________________________________ <br>- Restriction Digestion [1-16 h] <br>- PCR purification [1 h] <br>- Agarose Gel Electrophoresis [2 h] <br>MAT...) (top)
- 20:15, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Competent Cell Preparation (New page: <br>MAIN STEPS/TIME TABLE <br>________________________________________ <br>- Incubation [12-14 h] >>> Spread plate <br>- Incubation [12-14 h] >>> Liquid culture <br>- Competent cell prep...)
- 20:06, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Alkaline Phosphatasen (New page: <br>MATERIALS <br>________________________________________ <br>- Heat block <br>- Spin <br>- Linear DNA <br>- 10X reaction buffer for AP used in reaction <br>- FastAP™ Thermosensitiv...) (top)
