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User contributions
From 2010.igem.org
- 17:38, 27 October 2010 (diff | hist) N HKU-Hong Kong/26 October 2010 (New page: == Working schedule 26 October 2010 == Finally check wiki and type in the lab notebooks onto the website.)
- 17:31, 27 October 2010 (diff | hist) N HKU-Hong Kong/19 August 2010 (New page: == Working schedule 19 August 2010 == Enzyme digestion to identify the plasmids.)
- 17:29, 27 October 2010 (diff | hist) N HKU-Hong Kong/17 August 2010 (New page: == Working schedule 17 August 2010 == Plasmid extraction.)
- 17:28, 27 October 2010 (diff | hist) N HKU-Hong Kong/16 August 2010 (New page: == Working schedule 16 August 2010 == Pick up colonies and shake over night.)
- 17:27, 27 October 2010 (diff | hist) N HKU-Hong Kong/14 August 2010 (New page: == Working schedule 14 August 2010 == Colonies present, not pink. :) Colony PCR.)
- 17:25, 27 October 2010 (diff | hist) N HKU-Hong Kong/13 August 2010 (New page: == Working schedule 13 August 2010 == There are still no colonies. Use the newly ligated DNA to do transformation.)
- 17:24, 27 October 2010 (diff | hist) N HKU-Hong Kong/12 August 2010 (New page: == Working schedule 12 August 2010 == No colony. Repeat ligation and transformation.)
- 17:22, 27 October 2010 (diff | hist) N HKU-Hong Kong/11 August 2010 (New page: == Working schedule 11 August 2010 == Transform the ligation product.)
- 17:21, 27 October 2010 (diff | hist) N HKU-Hong Kong/10 August 2010 (New page: == Working schedule 10 August 2010 == Dissolve the linearized backbone in the gel slice. Ligation.)
- 17:20, 27 October 2010 (diff | hist) N HKU-Hong Kong/8 August 2010 (New page: == Working schedule 7 August 2010 == LacA PCR done. Purify the PCR product. Enzyme digestion)
- 17:18, 27 October 2010 (diff | hist) N HKU-Hong Kong/6 August 2010 (New page: == Working schedule 6 August 2010 == Backbone plasmids are cut. Separate the plasmid fragments using agarose gel.)
- 17:15, 27 October 2010 (diff | hist) N HKU-Hong Kong/5 August 2010 (New page: == Working schedule 5 August 2010 == There is band but not significant. Adjust condition and repeat.)
- 17:14, 27 October 2010 (diff | hist) N HKU-Hong Kong/4 August 2010 (New page: == Working schedule 4 August 2010 == Repeat PCR)
- 17:10, 27 October 2010 (diff | hist) N HKU-Hong Kong/2 August 2010 (New page: == Working schedule 2 August 2010 == Colony PCR with the lac A primers.)
- 17:08, 27 October 2010 (diff | hist) N HKU-Hong Kong/1 August 2010 (New page: == Working schedule 1 August 2010 == Plasmids were successfully extracted. Primers arrived. Bacterias were streaked.)
- 17:04, 27 October 2010 (diff | hist) N HKU-Hong Kong/31 July 2010 (New page: == Work Schedule 31 July 2010 == The bacteria stored in the fridge was taken out and plasmids were extracted. Somehow genomic DNA was present as contaminants.)
- 17:00, 27 October 2010 (diff | hist) N HKU-Hong Kong/29 July 2010 (New page: == Work Schedule 29 July 2010 == Pink colonies were seen. Pick them up and shake in warm room.)
- 16:48, 27 October 2010 (diff | hist) N HKU-Hong Kong/28 July 2010 (New page: == Work Schedule 28 July 2010 == No colonies! Transform the plasmids again using electronic method.)
- 16:44, 27 October 2010 (diff | hist) HKU-Hong Kong/27 July 2010 (→Work schedule 27 July 2010)
- 16:34, 27 October 2010 (diff | hist) Team:HKU-Hong Kong/Team (top)
- 16:28, 27 October 2010 (diff | hist) User:Hp1989 (→He Peng (Brian)) (top)
- 16:27, 27 October 2010 (diff | hist) User:Hp1989 (→He Peng (Brian))
- 16:23, 27 October 2010 (diff | hist) User:Hp1989 (→He Peng (Brian))
- 16:00, 27 October 2010 (diff | hist) N User:Hp1989 (New page: == He Peng (Brian) == *Email: hepeng@hku.hk/hepeng@ucla.edu *Address: 1104A, Morrison, Pokfulam, Hong Kong *Facebook: [http://www.facebook.com/peng.he1 add Brian])
- 07:34, 27 October 2010 (diff | hist) Team:HKU-Hong Kong/Team
- 07:19, 27 October 2010 (diff | hist) Team:HKU-Hong Kong/Team
- 17:33, 26 October 2010 (diff | hist) Team:HKU-Hong Kong/Parts (→Parts) (top)
- 17:27, 26 October 2010 (diff | hist) Team:HKU-Hong Kong/Parts (→Parts)
- 17:04, 26 October 2010 (diff | hist) Team:HKU-Hong Kong/Team
- 16:32, 26 October 2010 (diff | hist) Team:HKU-Hong Kong/Team
