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From 2010.igem.org

Duisrupt your cells using a French press style cell disruptor, keep all collecting parts cool to reduce protease activity.

Repeat 6x, pressure: 13 kPSI

Spin off, 5 minutes at 4000 rpm.

Discard supernatant.

Cook pellet in 2% sds

Spin down, 4000 rpm.

Cook pellet in 2% SDS

Wash 10x

Dry freeze