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From 2010.igem.org
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To view lengths of DNA, Orange G 6x was added 1:3 to 300ng miniprepped DNA and run against 5uL of Hyperladder I in the lanes of 1% UltraPure agarose gels in TAE buffer at 120V for 45 minutes.<br> | To view lengths of DNA, Orange G 6x was added 1:3 to 300ng miniprepped DNA and run against 5uL of Hyperladder I in the lanes of 1% UltraPure agarose gels in TAE buffer at 120V for 45 minutes.<br> | ||
<b>DNA Gel Extraction</b><br> | <b>DNA Gel Extraction</b><br> | ||
| - | To extract the DNA from the agarose gel, the | + | To extract the DNA from the agarose gel, the QIAquick Gel Extraction kit was used according to protocol.</td> |
</table> | </table> | ||
Revision as of 15:54, 10 October 2010
iGEM 2010
Undergraduates Instructors Advisors Fun Sponsors
Overview Toggle Phage Mammalian Summary Acknowledgements
Materials & Methods Biosafety Journal Club
Bacterial Construction Protocol
Bacterial Experimental Protocol
Phage western blot
Mammalian Cell Lines
Microfluidic stress
Materials
biobrick construction |
Antarctic Phosphatase To prevent recircularization of digested plasmids, we used NEB's Antarctic Phosphatase according to protocol. Gel Electrophoresis To view lengths of DNA, Orange G 6x was added 1:3 to 300ng miniprepped DNA and run against 5uL of Hyperladder I in the lanes of 1% UltraPure agarose gels in TAE buffer at 120V for 45 minutes. DNA Gel Extraction To extract the DNA from the agarose gel, the QIAquick Gel Extraction kit was used according to protocol. |
