Team:Yale/Our Project/Notebook/Week 5

From 2010.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 22: Line 22:
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 7">week 7</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 7">week 7</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 8">week 8</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 8">week 8</a></li>
 +
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 9">week 9</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
Line 130: Line 131:
<ul>
<ul>
<li>Needed more phsABC, so ran five PCR reactions to amplify it from plasmid pSB74. Reaction mixture contents were the same as the DMSO variant used on <a href="https://2010.igem.org/Team:Yale/Our_Project/Notebook/Week_4">6/30 </a>as was the thermocycler protocol (phs50). The results were then run on a gel</li>
<li>Needed more phsABC, so ran five PCR reactions to amplify it from plasmid pSB74. Reaction mixture contents were the same as the DMSO variant used on <a href="https://2010.igem.org/Team:Yale/Our_Project/Notebook/Week_4">6/30 </a>as was the thermocycler protocol (phs50). The results were then run on a gel</li>
-
       Gel of PCR amplified phsABC Extreme right lane:1 kb ladder, all other lanes PCR reaction mixtures and their spillover
+
<div align="center">
 +
<img src="https://static.igem.org/mediawiki/2010/a/ac/Yale-pcr-gel.jpg" />
 +
</div>
 +
       <div id="caption">Gel of PCR amplified phsABC Extreme right lane:1 kb ladder, all other lanes PCR reaction mixtures and their spillover</div>
        
        
<li>Needed chloramphenicol plates for pSB1C3-based constructs, so mixed 350 μL of 50 μg/μL chloramphenicol with an autoclaved solution of 7.5 g agar and 12.5 g LB in 500 mL H2O and poured plates. </li>
<li>Needed chloramphenicol plates for pSB1C3-based constructs, so mixed 350 μL of 50 μg/μL chloramphenicol with an autoclaved solution of 7.5 g agar and 12.5 g LB in 500 mL H2O and poured plates. </li>
Line 352: Line 356:
<li>Based on the results, all enzymes appeared to work, as they successfully created a linearized 3.2 kb fragment.</li>
<li>Based on the results, all enzymes appeared to work, as they successfully created a linearized 3.2 kb fragment.</li>
 +
<div align="center">
 +
<img src="https://static.igem.org/mediawiki/2010/9/93/Yale-enzyme-test.jpg" />
 +
</div>
 +
<i>Wetlab work for this day is also recorded on pages 54-57 of the hard copy lab notebook. </i>
<i>Wetlab work for this day is also recorded on pages 54-57 of the hard copy lab notebook. </i>
</ul>
</ul>

Latest revision as of 02:51, 28 October 2010

iGEM Yale

lab notebook: week 5 (7/5 -7/11)

  • Monday 7/5--Colony PCR of Ligation Attempt #2 Transformants
  • See more/less
  • Tuesday 7/6--Analysis of Results of Ligation Attempts 1 & 2
  • See more/less
  • Wednesday 7/7--Triple ligation attempt--promoter + thiosulfate reductase + terminator
  • See more/less
  • Thursday 7/8--Further ligation efforts
  • See more/less
  • Friday 7/9--Ligation attempt #4 to join phsABC and B0015 terminator.
  • See more/less
  • Saturday 7/10--Transformants from ligation attempts
  • See more/less
  • Sunday 7/11--Prep for copper growth assays of transformants
  • See more/less