Team:Yale/Our Project/Notebook/Week 2

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<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 7">week 7</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 7">week 7</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 8">week 8</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 8">week 8</a></li>
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<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 9">week 9</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
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<h4> Copper Growth Assay Work Continues </h4>
<h4> Copper Growth Assay Work Continues </h4>
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<img src="https://static.igem.org/mediawiki/2010/8/8f/Yale-growthfail.jpg" />
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<ul>
<ul>
<li>As the growth assay of <a href="https://2010.igem.org/Team:Yale/Our_Project/Notebook">6/11</a> showed uniformly poor growth even at the lowest copper concentrations (see Dh5alpha growth above), redid it with care not to let the liquid cultures overgrow, a possible source of the cultures' previous poor performance. (add plot)</li>
<li>As the growth assay of <a href="https://2010.igem.org/Team:Yale/Our_Project/Notebook">6/11</a> showed uniformly poor growth even at the lowest copper concentrations (see Dh5alpha growth above), redid it with care not to let the liquid cultures overgrow, a possible source of the cultures' previous poor performance. (add plot)</li>
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<h4> Results and Conclusions of Narrow Concentration Range Growth Assay of 6/14</h4>
<h4> Results and Conclusions of Narrow Concentration Range Growth Assay of 6/14</h4>
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<img src="https://static.igem.org/mediawiki/2010/d/d1/Yale-dh5alpha-narrow.jpg" />
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<ul>
<ul>
<li> Negative readings for the 4 mM samples suggest that there was some sort of irregularity with the blank solution, so will disregard those results. </li>
<li> Negative readings for the 4 mM samples suggest that there was some sort of irregularity with the blank solution, so will disregard those results. </li>
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*This plan was eventually altered, as promoter choices were altered and no light-inducible promoter was found. <br/>
*This plan was eventually altered, as promoter choices were altered and no light-inducible promoter was found. <br/>
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<img src="https://static.igem.org/mediawiki/2010/7/71/Yale-biobrick.jpg" />
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<b>BL21 copper growth assay analysis</b> <br/>
<b>BL21 copper growth assay analysis</b> <br/>
Retrieved the following data regarding BL21's growth in copper solution: <br/>
Retrieved the following data regarding BL21's growth in copper solution: <br/>
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It appears that BL21 is slightly more sensitive to copper than the other two strains, since 3 mM levels of copper(II) sulfate are enough to almost completely inhibit BL21's growth.
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<img src="https://static.igem.org/mediawiki/2010/f/f6/Yale-bl21.jpg" />
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It appears that BL21 is slightly more sensitive to copper than the other two strains, since 3 mM levels of copper(II) sulfate are enough to almost completely inhibit BL21's growth. <br/>
<i> The activities of this day are also recorded on page 15 of the hard copy lab notebook </i>
<i> The activities of this day are also recorded on page 15 of the hard copy lab notebook </i>
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Latest revision as of 02:49, 28 October 2010

iGEM Yale

lab notebook: week 2 (6/14-6/20)

  • Monday 6/14--Redo of 6/11 assay of bacterial growth within a narrow copper(II) concentrations after analysis of data showed uniformly poor growth.
  • See more/less
  • Tuesday 6/15--Results and analysis of the 6/14 narrow concentration range growth assay as well as planning for the creation of a standard iGEM plasmid bearing the thiosulfate reductase operon (phsABC).
  • See more/less
  • Wednesday 6/16--Checked on spotted cell survival assay, collected MOPS minimal media materials & started making component solutions
  • See more/less
  • Thursday 6/17--more minimal media work and meeting, started BL21 culture
  • See more/less
  • Friday 6/18--Arrival of plasmid pSB74, transformation of Biobricks, & copper growth assays for BL21 strain.
  • See more/less
  • Saturday 6/19--Redo of Biobrick transformations & analysis of BL21 copper growth assay
  • See more/less
  • Sunday 6/20--Observed growth of all transformants from 6/19, so used them and the culture containing pSB74 to inoculate 5 mL liquid cultures in LB with ampicillin. Left to grow overnight on shaker at 37˚C for miniprep the following morning.
    The activities of this day are also recorded on page 16 of the hard copy lab notebook