Team:Warsaw/Calendar-Stage1/14 September 2010

From 2010.igem.org

(Difference between revisions)
Qba (Talk | contribs)
(New page: <html> <script type="text/javascript"> ActiveTab='WetLab'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <!-- do not edit above me! --> <h4>Kuba</h4> <p align="...)
Newer edit →

Revision as of 14:44, 25 October 2010

Example Tabs

Kuba

Directed mutagenesis was preformed on pSB-MinC in order to remove an additional PstI site. Two primers were used: MinCmutF (TAATTGCAGTCGCGCCGC) and MinCmutR (GTCGAAAACGCTTTGACCG). PCR was run with Yellow Pfu Polimerase (Eurex) in a standard reaction mix.

PCR conditions were as follows:

  • initial denaturation 95 oC; 3'
  • denaturation 95 oC; 30 s
  • annealing 51 oC; 30 s
  • elongation 72 oC<; 7'/li>
  • x 30 cycles
  • 72 oC; 10'

Retrieved from "http://2010.igem.org/Team:Warsaw/Calendar-Stage1/14_September_2010"