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- | <br> 10.09.2010 Friday
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- | <br><br>Note: We're not satisfied with the results, because of some problems with the timetable of usage of the cytometer
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- | <br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div>
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- | <br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)
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- | <br><div class="note"> Previous day: Set up liquid cultures of different types of E.coli [Ania P.]</div>
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- | <br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)
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- | <br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div>
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- | <br>1. Incubation of Hela cells with different E.coli types.
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- | <br>2. Preparation of probes with incubated cells and examining them in flow cytometer.
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- | <br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)<br>
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- | <br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div>
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- | <br>
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- | <br /><strong>legend:</strong>
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- | <hr />
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- | <br />E.coli constructs:
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- | <br />G- GFP
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- | <br />I- Invasin+GFP
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- | <br />J- J23102+Listeriolysin O+Invasin+GFP
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- | <br />A- pAraC+Listeriolysin O+Invasin+GFP
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- | <br /><strong>Hela cells with bacteria:</strong>
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- | <br />ML.009- without bacteriaN
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- | <br />ML.010- TOP 10 (50)
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- | <br />ML.003- G (25)
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- | <br />ML.004- I (25)
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- | <br />ML.008- I (50)
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- | <br />ML.011- G (50)
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- | <br />10-09-2010.001- J (50)
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- | <br />10-09-2010.002- A (50)
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- | <br />10-09-2010.003- J (25)
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- | <hr />
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- | Data from the flow cytometer processed by Cell Quest:
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- | <br><br><br/>
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- | <img src="https://static.igem.org/mediawiki/2010/7/71/Bacto.10.09_01.png" width="450px" />
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- | <img src="https://static.igem.org/mediawiki/2010/5/59/Bacto.10.09_02.png" width="800px" />
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- | <img src="https://static.igem.org/mediawiki/2010/1/1a/Bacto.10.09_03.png" width="800px" />
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