Team:WITS-South Africa

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Revision as of 18:48, 17 October 2010

Our Project - Lactoguard

So what is "Lactoguard?"

It is a whole-cell biosensor for the immediate, in vivo detection of Human Papillomavirus (HPV), and thus cervical cancer, in the form of an engineered commensal vaginal bacterium. It is a safe, low-cost and easy-to-administer machine intended primarily for women in resource-poor settings.

The link between HPV and cervical cancer is well-established; and while there are vaccines available against HPV, they are limited (due issues around cost and accessibility) in their ability to prevent disease burden in developing countries, where 80% of cervical cancer deaths occur annually.

This inspired the search for a method of detecting the virus that will alert an infected individual that they have been exposed. The machine also has the potential to be further adapted to neutralise the virus and prevent the infection from spreading.

The chassis of our machine, Lactobacillus gasseri, is a predominant commensal vaginal microbe and is ideally situated to detect and deter HPV. Interbacterial communication is achieved by quorum sensing, which has been studied in detail in various organisms such as gram positive bacilli and thus we have elected to use one of these pathways as the means of propagating the response signal throughout the population. Each bacterium will produce a visible reporter on receipt of the signal which the infected woman would be able to detect without medical assistance shortly after infection.

Meet the team

Our team consists of students from various disciplines such as molecular biology, mathematics, engineering and philosophy. Whilst the biologists will focus on building the machine in the wetlab, the mathematicians and engineers will model its predicted behaviour and effect in vivo whilst the philosopher will focus on the ethics of creating such a machine for medical application.

Where are you viewing from?

The map below indicates where the people who view our wiki are from. This is a cool way to see how far our message is spreading!

To find out more about HPV and cervical cancer click here!

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-Our Project - Lactoguard
+== Our Project - Lactoguard ==
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-The development of a whole-cell biosensor for the immediate, in vivo detection of '''Human Papillomavirus (HPV)''', in the form of an engineered commensal vaginal bacterium. This mechanism would be safe, low-cost and easily administrable; and is intended primarily for women in resource-poor settings.
+[[Image:Bacteria.jpg|200px|left]]
+So what is "Lactoguard?"
+It is a whole-cell biosensor for the immediate, in vivo detection of '''Human Papillomavirus (HPV)''', and thus cervical cancer, in the form of an engineered commensal vaginal bacterium. It is a safe, low-cost and easy-to-administer machine intended primarily for women in resource-poor settings.
+The link between HPV and cervical cancer is well-established; and while there are vaccines available against HPV, they are limited (due issues around cost and accessibility) in their ability to prevent disease burden in developing countries, where 80% of cervical cancer deaths occur annually.
-The link between HPV and cervical cancer is well-established. Although much progress has been made in developing a vaccine, the vaccines that are currently available have some limitations (mainly due to high cost and inadequate accessibility) in their ability to prevent disease burden in developing countries, where 80% of cervical cancer deaths occur annually.
+This inspired the search for a method of detecting the virus that will alert an infected individual that they have been exposed. The machine also has the potential to be further adapted to neutralise the virus and prevent the infection from spreading.
-This inspired the search for a method of detecting the virus that will alert the infected individual in a discreet yet persistent manner. The machine would also have the potential to be further adapted to have the ability to neutralise the virus and prevent the infection from spreading. Basically, the machine that we wish to design will be able to detect an infectious agent and inform neighbouring bacteria - as well as the host - as to the presence of that pathogen.
+The chassis of our machine, <i>Lactobacillus gasseri</i>, is a predominant commensal vaginal microbe and is ideally situated to detect and deter HPV. Interbacterial communication is achieved by quorum sensing, which has been studied in detail in various organisms such as gram positive bacilli and thus we have elected to use one of these pathways as the means of propagating the response signal throughout the population. Each bacterium will produce a visible reporter on receipt of the signal which the infected woman would be able to detect without medical assistance shortly after infection.
-The chassis of the machine, <i>Lactobacillus gasseri</i>, is a predominant commensal vaginal microbe and is ideally situated to detect and deter HPV. Interbacterial communication is achieved by quorum sensing, which has been studied in detail in various organisms such as gram positive bacilli and thus we have elected to use one of these pathways as the means of propagating the response signal throughout the population. Each bacterium will produce a visible reporter on receipt of the signal which the infected woman would be able to detect without medical assistance shortly after infection.
+== Meet the team ==
+[[Image:wits_team.jpg|220px|right]]
 Our team consists of students from various disciplines such as molecular biology, mathematics, engineering and philosophy. Whilst the biologists will focus on building the machine in the wetlab, the mathematicians and engineers will model its predicted behaviour and effect in vivo whilst the philosopher will focus on the ethics of creating such a machine for medical application.