Team:Uppsala-SwedenWeek2

From 2010.igem.org

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(New page: {{Template:Uppsala}}<!--Do not remove the first and last lines in this page!--> == Week-2 == == Preparation for getting the bricks: == (1)Competent cell preparation (2)Transformation of...)
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== Week-2 ==
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== Week-2 (Investigation of Singing E.coli project)==
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== Preparation for getting the bricks: ==
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We think the singing E.coli project will be an innovative, valuable and interesting topic for 2010 iGEM competition. Its concept could be applied to some environmental project, such as removal and detection of specific heavy metals. However, concept only is not good enough for practical work and we decided to have more digging on the scientific background for it.
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(1)Competent cell preparation
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The first thing come up in our minds is to express molecular magnetic protein in the E.coli. After searching some online sources, we found some natural protein have magnetic properties, such as Magnetoferritin. Ferritin is a spherical polypeptide cage capturing a nano-sized core of ferromagnetic iron oxide magenetite. It could be synthesized by in vitro accoding to F. Meldrum team in 1992. [http://adsabs.harvard.edu/abs/1992Sci...257..522M i] However, their synthesis method is based on horse spleen ferritin under controlled reconstitution condition. It would be quite tricky to synthesize the animal protein in bacterial cells. Another magnetic protein, metallothionein, which is an antioxidant in mammalian, was excluded from the consideration for the same reason.
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(2)Transformation of the Biobricks into DH5-alpha competent cells
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The second idea is to insert metal-ion attractive group from magnetotactic bacteria into the fusion protein in E.coli. But the genes of magnetic group are not well defined which might cause quite a big problem during cloning.
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(3)Verificiation of the lengths of the biobricks by Gel Electrophoresis after PCR.
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The third idea is to insert the chemotactic (thermotactic) protein in magentotactic bacteria. However, culturing of those bacteria might be a problem. Our group searched culturing methods for 12 species of most common magentotactic bacteria. We checked DSMZ which sells some magnetotactic species [http://www.dsmz.de/microorganisms/html/strains/strain.dsm006361.html ii] and found that kind of organisms are quite difficult to cultivate, complex medium, anaerobic or micro-aerophilic. Moreover, as limited research is done on magetotactic bacteria, the available genetic systems might get problem when introducing new genes in these bacteria.
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== Details of the biobricks we are using: ==
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So we finally decided to give up this interesting but a bit intangible idea and working on the living biological clock project.
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  <p class=MsoNormal align=center style='text-align:center'><b><span
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  style='font-size:14.0pt;font-family:Calibri;color:black'>Biobricks details</span></b></p>
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Part         BBa_</span></b></p>
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Name</span></b></p>
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Length (bp)</span></b></p>
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Plasmid     Length</span></b></p>
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  <td width=51 valign=bottom style='width:38.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Total Length</span></b></p>
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  <td width=55 valign=bottom style='width:41.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Plasmid</span></b></p>
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  <td width=60 valign=bottom style='width:45.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Plasmid number</span></b></p>
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  <td width=35 valign=bottom style='width:26.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Plate</span></b></p>
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Well</span></b></p>
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  <td width=65 valign=bottom style='width:49.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Antibiotics</span></b></p>
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  <td width=75 valign=bottom style='width:56.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Sequencing</span></b></p>
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  <td width=104 valign=bottom style='width:78.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Quality (Gel, Q, P)</span></b></p>
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  </td>
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  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
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  <p class=MsoNormal><b><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Function</span></b></p>
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  </td>
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</tr>
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<tr style='height:15.0pt'>
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  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>F 2621</span></p>
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  </td>
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  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>F2</span></p>
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  </td>
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  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>1158</span></p>
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  </td>
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  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
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  </td>
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  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>3237</span></p>
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  </td>
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  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>PSB1A2</span></p>
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  </td>
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  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>All</span></p>
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  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>21F</span></p>
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  </td>
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  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Amp</span></p>
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  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Confirmed</span></p>
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  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Bad, OK, OK</span></p>
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  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Sensor</span></p>
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</tr>
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<tr style='height:15.0pt'>
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  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>F 1610</span></p>
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  </td>
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  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>F1</span></p>
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  </td>
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  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>798</span></p>
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  </td>
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  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>3189</span></p>
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  </td>
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  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>3987</span></p>
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  </td>
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  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>pSB1Ak3</span></p>
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  </td>
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  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>AHL Relay</span></p>
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  </td>
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  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
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  <p class=MsoNormal align=right style='text-align:right'><span
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  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
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  </td>
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  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>24G</span></p>
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  </td>
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  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Amp &amp; Km</span></p>
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  </td>
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  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>Confirmed</span></p>
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  </td>
+
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  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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+
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>OK, OK, OK</span></p>
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  </td>
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  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>AHL generator</span></p>
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  </td>
+
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</tr>
+
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<tr style='height:26.25pt'>
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  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
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  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
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  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
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  color:black'>I 746350</span></p>
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  </td>
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  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
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+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F17</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>237</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2316</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>1 &amp; 4</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>12C</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Partially</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Activator in sensitivity tuner</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:26.25pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I 746352</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F4</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>264</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><b><span
+
-
  style='font-size:10.0pt;font-family:Arial;color:black'>2079</span></b></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2343</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>2,3,5 &amp; 6</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>12G</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Partially</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Activator in sensitivity tuner</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:15.0pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>B0015</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F5</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>129</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>3189</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>3318</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1AK3</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>All</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>23L</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp &amp; Km</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Terminator</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:26.25pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I746360</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F6</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>91</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2170</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>1 &amp; 4</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>12I</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Lower level promotor</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:26.25pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I746364</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F7</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>93</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2172</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>2 &amp; 5</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>12O</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Lower level promotor</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:26.25pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I746365</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F8</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>92</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2171</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>3 &amp; 6</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>14A</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Inconsistent</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Lower level promotor</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:15.0pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Q04510</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F9</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>987</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>4425</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>5412</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB2K3</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>All</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>18B</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Km</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, Low, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Inverter</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:26.25pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>P0412</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F10</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1308</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>3387</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>All</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>3</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>6P</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Lower level repressor </span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:26.25pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I746361</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F11</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>92</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2171</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>1,2,4 &amp; 5</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>12K</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Higher level promotor</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:26.25pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>R0010</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F12</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>200</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2279</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>4,5 &amp; 6</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>1D</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:26.25pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Regulatory promotor</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:15.0pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I0460 </span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F16</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>969</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>3189</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>4158</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1AK3</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>4,5 &amp; 6</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>24I</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp &amp; Km</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Partially</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, OK, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>AiiA generator</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:15.0pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I13504</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F13</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>875</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2954</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>1 (GFP)</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>22I</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, High, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>GFP Generator</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:15.0pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>I13507</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F14</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>861</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2940</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>2 (RFP)</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>22O</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, High, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>RFP Generator</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:15.0pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>E0430</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F15</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>878</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2957</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>3 (YFP)</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>8I</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, High, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>YFP Generator</span></p>
+
-
  </td>
+
-
</tr>
+
-
<tr style='height:15.0pt'>
+
-
  <td width=95 valign=bottom style='width:71.0pt;border:solid windowtext 1.0pt;
+
-
  border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>B0034</span></p>
+
-
  </td>
+
-
  <td width=40 nowrap valign=bottom style='width:30.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>F3</span></p>
+
-
  </td>
+
-
  <td width=45 nowrap valign=bottom style='width:34.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>12</span></p>
+
-
  </td>
+
-
  <td width=49 nowrap valign=bottom style='width:37.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2079</span></p>
+
-
  </td>
+
-
  <td width=51 nowrap valign=bottom style='width:38.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>2091</span></p>
+
-
  </td>
+
-
  <td width=55 nowrap valign=bottom style='width:41.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>pSB1A2</span></p>
+
-
  </td>
+
-
  <td width=60 nowrap valign=bottom style='width:45.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>4,5 &amp; 6</span></p>
+
-
  </td>
+
-
  <td width=35 nowrap valign=bottom style='width:26.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal align=right style='text-align:right'><span
+
-
  style='font-size:10.0pt;font-family:Calibri;color:black'>1</span></p>
+
-
  </td>
+
-
  <td width=32 nowrap valign=bottom style='width:24.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>2M</span></p>
+
-
  </td>
+
-
  <td width=65 nowrap valign=bottom style='width:49.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Amp</span></p>
+
-
  </td>
+
-
  <td width=75 nowrap valign=bottom style='width:56.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>Confirmed</span></p>
+
-
  </td>
+
-
  <td width=104 nowrap valign=bottom style='width:78.0pt;border-top:none;
+
-
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>OK, High, OK</span></p>
+
-
  </td>
+
-
  <td width=116 valign=bottom style='width:87.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
-
  padding:0cm 3.5pt 0cm 3.5pt;height:15.0pt'>
+
-
  <p class=MsoNormal><span style='font-size:10.0pt;font-family:Calibri;
+
-
  color:black'>RBS</span></p>
+
-
  </td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
== Gel image of the biobricks after confirmation of lengths: ==
+
-
 
+
-
[[Image:20100805 biobrick 20100805.jpg|600px|Biobricks length confirmation]]
+
-
 
+
-
 
+
-
 
+
-
    * Outcome:We confirmed the lengths of almost all the transformed cells of the required biobricks to be correct as per above gel image.
+

Latest revision as of 09:24, 23 September 2010

Week-2 (Investigation of Singing E.coli project)

We think the singing E.coli project will be an innovative, valuable and interesting topic for 2010 iGEM competition. Its concept could be applied to some environmental project, such as removal and detection of specific heavy metals. However, concept only is not good enough for practical work and we decided to have more digging on the scientific background for it.

The first thing come up in our minds is to express molecular magnetic protein in the E.coli. After searching some online sources, we found some natural protein have magnetic properties, such as Magnetoferritin. Ferritin is a spherical polypeptide cage capturing a nano-sized core of ferromagnetic iron oxide magenetite. It could be synthesized by in vitro accoding to F. Meldrum team in 1992. [http://adsabs.harvard.edu/abs/1992Sci...257..522M i] However, their synthesis method is based on horse spleen ferritin under controlled reconstitution condition. It would be quite tricky to synthesize the animal protein in bacterial cells. Another magnetic protein, metallothionein, which is an antioxidant in mammalian, was excluded from the consideration for the same reason.

The second idea is to insert metal-ion attractive group from magnetotactic bacteria into the fusion protein in E.coli. But the genes of magnetic group are not well defined which might cause quite a big problem during cloning.

The third idea is to insert the chemotactic (thermotactic) protein in magentotactic bacteria. However, culturing of those bacteria might be a problem. Our group searched culturing methods for 12 species of most common magentotactic bacteria. We checked DSMZ which sells some magnetotactic species [http://www.dsmz.de/microorganisms/html/strains/strain.dsm006361.html ii] and found that kind of organisms are quite difficult to cultivate, complex medium, anaerobic or micro-aerophilic. Moreover, as limited research is done on magetotactic bacteria, the available genetic systems might get problem when introducing new genes in these bacteria.

So we finally decided to give up this interesting but a bit intangible idea and working on the living biological clock project.

Retrieved from "http://2010.igem.org/Team:Uppsala-SwedenWeek2"