Team:Uppsala-SwedenWeek16
From 2010.igem.org
Week-16
Start to apply high fidelity PCR due the construct are getting larger and the regular Taq polymerase may not perform as well as the Phusion Polymerase at this length. Based on the company´s manual, we calculated the
|
Temperatur |
Time |
Cycle |
Initial denaturation |
98 |
30 s |
1 |
Denaturation |
98 |
10 |
30 |
Annealing |
55.3 |
10s |
30 |
Extension |
72 |
1 min 45 s |
30 |
Final extension |
72 |
5 min |
1 |
|
4 |
forever |
|