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Team:Uppsala-SwedenWeek16
From 2010.igem.org
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Revision as of 19:40, 26 October 2010
Week-16
Start to apply high fidelity PCR due the construct are getting larger and the regular Taq polymerase may not perform as well as the Phusion Polymerase at this length. Based on the company´s manual, we calculated the
|
|
Temperatur |
Time |
Cycle |
|
Initial denaturation |
98 |
30 s |
1 |
|
Denaturation |
98 |
10 |
30 |
|
Annealing |
55.3 |
10s |
30 |
|
Extension |
72 |
1 min 45 s |
30 |
|
Final extension |
72 |
5 min |
1 |
|
|
4 |
forever |
|
The purpose of this was to get more reliable band when doing a PCR and running the gel.
