Team:UTDallas/Safety

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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{{Template:UTDallasTop}}
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==Safety==
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Please use this page to answer the safety questions posed on the [[Safety | safety page]].
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace. 
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Safety Questions: <br>
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'''1. Would any of your project ideas raise safety issues in terms of researcher, public, or environmental safety?''' <br>
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*Materials that pose a safety risk (i.e. cell cultures, ethidium bromide, UV transilluminator, etc) are handled according to standard lab safety protocol and Materials Safety Data Sheets. Materials containing safety hazards are disposed in separate containers with “biohazard” designations. Appropriate protective gear, such as gloves, is worn at all times. Our bacterial chassis, E. coli DH5α, is disabled to where it is nonpathogenic and cannot survive outside of lab conditions. In addition, all exposed counters are disinfected using 70% ethanol after each use of the lab. All lab equipment is thoroughly cleaned and autoclaved after use and access to the lab is limited by cardkey.
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'''2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?''' <br>
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*We worked with genes encoding known properties and utilized safety measures to ensure that biohazardous materials including antibiotic-resistant cells are contained within the lab and are appropriately disposed.  Therefore, we assess that none of our submitted parts raise safety issues.
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'''3. Is there a local biosafety group, committee, or review board at your institution?''' <br>
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*UT Dallas has an Institutional Biosafety Committee that manages all safety responsibilities under NIH “Guidelines for Research Involving Recombinant DNA Molecules”. Throughout the course of this work, we ensured that all lab activity respected safety measures.
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'''4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?''' <br>
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*The iGEM plasmid backbone could be programmed to include an "inactivation mechanism" whose induction can be used to regulate the activity of cells endowed with BioBrick parts. Disseminating pertinent safety information through the Registry is a practical alternative to engineering biosafety measures into parts, devices and systems.
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:UTDallas_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:UTDallas_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:UTDallas | Team Example]]
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:UTDallas|Home]]
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!align="center"|[[Team:UTDallas/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=UTDallas Official Team Profile]
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!align="center"|[[Team:UTDallas/Project|Project]]
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!align="center"|[[Team:UTDallas/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UTDallas/Modeling|Modeling]]
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!align="center"|[[Team:UTDallas/Notebook|Notebook]]
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!align="center"|[[Team:UTDallas/Safety|Safety]]
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==Safety==
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Please use this page to answer the safety questions posed on the [[Safety | safety page]].
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Latest revision as of 02:58, 28 October 2010


Safety

Please use this page to answer the safety questions posed on the safety page.

Safety Questions:
1. Would any of your project ideas raise safety issues in terms of researcher, public, or environmental safety?

  • Materials that pose a safety risk (i.e. cell cultures, ethidium bromide, UV transilluminator, etc) are handled according to standard lab safety protocol and Materials Safety Data Sheets. Materials containing safety hazards are disposed in separate containers with “biohazard” designations. Appropriate protective gear, such as gloves, is worn at all times. Our bacterial chassis, E. coli DH5α, is disabled to where it is nonpathogenic and cannot survive outside of lab conditions. In addition, all exposed counters are disinfected using 70% ethanol after each use of the lab. All lab equipment is thoroughly cleaned and autoclaved after use and access to the lab is limited by cardkey.

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?

  • We worked with genes encoding known properties and utilized safety measures to ensure that biohazardous materials including antibiotic-resistant cells are contained within the lab and are appropriately disposed. Therefore, we assess that none of our submitted parts raise safety issues.

3. Is there a local biosafety group, committee, or review board at your institution?

  • UT Dallas has an Institutional Biosafety Committee that manages all safety responsibilities under NIH “Guidelines for Research Involving Recombinant DNA Molecules”. Throughout the course of this work, we ensured that all lab activity respected safety measures.

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

  • The iGEM plasmid backbone could be programmed to include an "inactivation mechanism" whose induction can be used to regulate the activity of cells endowed with BioBrick parts. Disseminating pertinent safety information through the Registry is a practical alternative to engineering biosafety measures into parts, devices and systems.