Team:USTC/Notebook/Meetings/16 September 2010

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Jocelyn:

colony PCR to test pduN.

colony PCR before miniprep ×40

double digest of ABJKTUV in promoter and pduN.

storage of bacterial ×36

Hilde:

Fastdigest cutting: pduABJKNTUV(E,X)

Ligation of Promotor-pduABJKNTUV

Transformation of ligation produce

Coating of Promotor-pduABJKNTUV onto Cl+ plate

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