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Team:UPO-Sevilla/Notebook/09 08
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Revision as of 16:40, 17 October 2010
September, 08th
Production Team
David Caballero. We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.
Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.
Assembly Team
Transformation of E. coli DH5-alfa with the products of ligations, and we spread in selective plates.
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