Team:UPO-Sevilla/Notebook/08 06

From 2010.igem.org

Revision as of 17:18, 13 October 2010 by Lepavgom (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

August, 6th

Production Team

Paola Gallardo. Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We run an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.

David Caballero. We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps are: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.

Assembly Team

Minpreps using inocula we put yesterday. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 & UPO11+2.

Return to Notebook

Footer