Team:UPO-Sevilla/Notebook/08 03


Revision as of 22:48, 26 October 2010 by Efercac (Talk | contribs)

August, 3rd

Production Team

Paola Gallardo. We digest fecI-fecR with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 pDC) and ligate it with the vector C3L (chloramphenicol).

Assembly Team

Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread on LB+Tc plates.

Go to 2nd of August Go to 4th of August
Return to Notebook