Team:UNIPV-Pavia/Freezer

From 2010.igem.org

(Difference between revisions)
Line 26: Line 26:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB2K3
+
<partinfo>pSB2K3</partinfo>
</td><td>
</td><td>
-
Seq ok(MIT); orange pellet when contained in pSB2K3
+
Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 43: Line 43:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
J63009 (Amp)
+
<partinfo>J63009</partinfo> (Amp)
</td><td>
</td><td>
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
Line 62: Line 62:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Seq ok(MIT)
Seq ok(MIT)
Line 80: Line 80:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1A1
+
<partinfo>pSB1A1</partinfo>
</td><td>
</td><td>
Seq ok(MIT 2009); length ok
Seq ok(MIT 2009); length ok
Line 97: Line 97:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1A2
+
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
Seq ok(MIT)
Seq ok(MIT)
Line 115: Line 115:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1A2
+
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
Seq ok(MIT); length ok
Seq ok(MIT); length ok
Line 132: Line 132:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1A2
+
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
Line 150: Line 150:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Wrong clone
Wrong clone
Line 168: Line 168:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Seq ok(BMR); length ok
Seq ok(BMR); length ok
Line 177: Line 177:
<tr>
<tr>
<td>
<td>
-
I1-1 (not used)
+
I1-1<br/>(not used)
</td><td>
</td><td>
<partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)
<partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)
Line 185: Line 185:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
J63009 (Amp)
+
<partinfo>J63009</partinfo> (Amp)
</td><td>
</td><td>
not sequenced, length ok
not sequenced, length ok
Line 202: Line 202:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
J63009 (Amp)
+
<partinfo>J63009</partinfo> (Amp)
</td><td>
</td><td>
Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
Line 221: Line 221:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Seq ok(BMR); Cm resistance ok; length ok.
Seq ok(BMR); Cm resistance ok; length ok.
Line 231: Line 231:
<tr>
<tr>
<td>
<td>
-
I2-2 (not used)
+
I2-2<br/>(not used)
</td><td>
</td><td>
<partinfo>BBa_P1004</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td>
<partinfo>BBa_P1004</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td>
Line 239: Line 239:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
not sequenced; Cm resistance ok
not sequenced; Cm resistance ok
Line 257: Line 257:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
Line 274: Line 274:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Wrong clone
Wrong clone
Line 283: Line 283:
<tr>
<tr>
<td>
<td>
-
I4-1 (not used)
+
I4-1<br/>(not used)
</td><td>
</td><td>
<partinfo>BBa_K165037</partinfo> (S-P)<br/>+<br/>I1 (X-P)</td>
<partinfo>BBa_K165037</partinfo> (S-P)<br/>+<br/>I1 (X-P)</td>
Line 291: Line 291:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
not sequenced; length ok
not sequenced; length ok
Line 308: Line 308:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1AK3
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Seq ok(BMR); length ok
Seq ok(BMR); length ok
Line 325: Line 325:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1A2
+
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
Seq ok(BMR); Cm resistance ok; length ok
Seq ok(BMR); Cm resistance ok; length ok
Line 342: Line 342:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1A3
+
<partinfo>pSB1A3</partinfo>
</td><td>
</td><td>
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
Line 351: Line 351:
<tr>
<tr>
<td>
<td>
-
I6-3 (not used)
+
I6-3<br/>(not used)
</td><td>
</td><td>
I3 (E-S)<br>+<br><partinfo>BBa_T9002</partinfo> (E-X)</td>
I3 (E-S)<br>+<br><partinfo>BBa_T9002</partinfo> (E-X)</td>
Line 359: Line 359:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
pSB1A3
+
<partinfo>pSB1A3</partinfo>
 +
</td><td>
 +
not sequenced
 +
</td>
 +
<td></td><td></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
I7<br/>
 +
</td><td>
 +
<partinfo>BBa_J23118</partinfo> (E-S)<br/>+<br/>I6-2 (E-X)</td>
 +
<td>
 +
23/06/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo>
 +
</td><td>
 +
not sequenced
 +
</td>
 +
<td></td><td></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
I8<br/>
 +
</td><td>
 +
<partinfo>BBa_J23110</partinfo> (E-S)<br/>+<br/>I6-2 (E-X)</td>
 +
<td>
 +
23/06/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo>
 +
</td><td>
 +
not sequenced
 +
</td>
 +
<td></td><td></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
I9<br/>
 +
</td><td>
 +
<partinfo>BBa_J23111</partinfo> (E-S)<br/>+<br/>I6-2 (E-X)</td>
 +
<td>
 +
23/06/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo>
 +
</td><td>
 +
not sequenced
 +
</td>
 +
<td></td><td></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
I10<br/>
 +
</td><td>
 +
<partinfo>BBa_J23116</partinfo> (E-S)<br/>+<br/>I6-2 (E-X)</td>
 +
<td>
 +
23/06/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo>
</td><td>
</td><td>
not sequenced
not sequenced

Revision as of 14:08, 24 June 2010
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Freezer Management



Biobrick or
ligation name		DescriptionCreation dateStrainVectorQuality controlLengthStore

<partinfo>BBa_E2050</partinfo>

mOrange, 744bp

08/06/2010

E. coli DH5-alpha

<partinfo>pSB2K3</partinfo>

Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator, 253 bp (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

<partinfo>BBa_K165037</partinfo>

tef2 promoter, 403bp

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(MIT)

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette, 769 bp

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A1</partinfo>

Seq ok(MIT 2009); length ok

<partinfo>BBa_J61001</partinfo>

R6K origin, 406bp

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(MIT)

<partinfo>BBa_K081008</partinfo>

RBS-luxI, 664bp

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(MIT); length ok

<partinfo>BBa_K125500</partinfo>

GFP fusion brick, 718bp (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

I0-1

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Wrong clone

I0-2

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); length ok

I1-1
(not used)

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

not sequenced, length ok

I1-2

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok

I2-1

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); Cm resistance ok; length ok.

I2-2
(not used)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

not sequenced; Cm resistance ok

I3-1

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok

I3-2

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Wrong clone

I4-1
(not used)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

not sequenced; length ok

I4-2

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); length ok

I5

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(BMR); Cm resistance ok; length ok

I6-2

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok

I6-3
(not used)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

not sequenced

I7

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6-2 (E-X)

23/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

not sequenced

I8

 <partinfo>BBa_J23110</partinfo> (E-S)
+
I6-2 (E-X)

23/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

not sequenced

I9

 <partinfo>BBa_J23111</partinfo> (E-S)
+
I6-2 (E-X)

23/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

not sequenced

I10

 <partinfo>BBa_J23116</partinfo> (E-S)
+
I6-2 (E-X)

23/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

not sequenced

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Freezer"