Team:UNIPV-Pavia/Freezer

From 2010.igem.org

(Difference between revisions)
m
Line 736: Line 736:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I20-A</font>
+
<font class="BBok">I20</font>
<br/>
<br/>
(it was I20M-1)
(it was I20M-1)
Line 764: Line 764:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I20-B</font>
+
<font class="BBok">I21</font>
<br/>
<br/>
-
(it was I20A-1)
+
(it was I21-4)
</td>
</td>
<td>
<td>
-
Pha-10S-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana5#July.2C_29th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
+
Pha-SS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
-
</td>
+
-
<td>
+
-
13/08/2010
+
</td>
</td>
<td>
<td>
 +
10/08/2010
 +
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
-
</td>
+
</td><td>
-
<td>
+
<partinfo>pSB1A3</partinfo>
-
<partinfo>pSB1AK3</partinfo>
+
</td><td>
-
</td>
+
-
<td>
+
Length ok.
Length ok.
</td>  
</td>  
Line 792: Line 789:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I21-A</font>
+
<font class="BBok">I22</font>
<br/>
<br/>
-
(it was I21M-3)
+
(it was I22-1)
</td>
</td>
<td>
<td>
-
Pha-SS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
+
<partinfo>BBa_K081009</partinfo> (E-S) <br/>+<br/> <partinfo>BBa_B0015</partinfo> (E-X)
</td>
</td>
<td>
<td>
-
13/08/2010
+
10/08/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
Line 809: Line 806:
</td>  
</td>  
<td>
<td>
-
576 bp
+
825 bp
</td>
</td>
<td>Ligations 2010</td>
<td>Ligations 2010</td>
Line 817: Line 814:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I21-B</font>
+
<font class="BBok">I23</font>
<br/>
<br/>
-
(it was I21-4)
+
(it was I23-1)
</td>
</td>
<td>
<td>
-
Pha-SS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
+
<partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I3 (X-P)
</td>
</td>
<td>
<td>
Line 829: Line 826:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
<partinfo>pSB1A3</partinfo>
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
Length ok.
Length ok.
</td>  
</td>  
<td>
<td>
-
576 bp
+
725 bp
</td>
</td>
<td>Ligations 2010</td>
<td>Ligations 2010</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I21-C</font>
+
<font class="BBok">I24</font>
<br/>
<br/>
-
(it was I21A-7)
+
(it was I24-1)
</td>
</td>
<td>
<td>
-
Pha-SS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
+
<partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I22 (X-P)
</td>
</td>
<td>
<td>
Line 852: Line 850:
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
-
</td><td>
+
</td>
 +
<td>
<partinfo>pSB1AK3</partinfo>
<partinfo>pSB1AK3</partinfo>
-
</td><td>
+
</td>
 +
<td>
Length ok.
Length ok.
</td>  
</td>  
<td>
<td>
-
576 bp
+
886 bp
</td>
</td>
<td>Ligations 2010</td>
<td>Ligations 2010</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I22</font>
+
<font class="BBok">I26</font>
<br/>
<br/>
-
(it was I22-1)
+
(it was I26-2)
</td>
</td>
<td>
<td>
-
<partinfo>BBa_K081009</partinfo> (E-S) <br/>+<br/> <partinfo>BBa_B0015</partinfo> (E-X)
+
<partinfo>BBa_K105012</partinfo> (S-P) <br/>+<br/> I21-B (X-P)
</td>
</td>
<td>
<td>
-
10/08/2010
+
13/08/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
-
</td><td>
+
</td>
-
<partinfo>pSB1AK3</partinfo>
+
<td>
-
</td><td>
+
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
Length ok.
Length ok.
</td>  
</td>  
<td>
<td>
-
825 bp
+
612 bp
</td>
</td>
<td>Ligations 2010</td>
<td>Ligations 2010</td>
Line 890: Line 893:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I23</font>
+
<font class="BBok">I29</font>
<br/>
<br/>
-
(it was I23-1)
+
(it was ??)
</td>
</td>
<td>
<td>
-
<partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I3 (X-P)
+
<partinfo>BBa_T9002</partinfo> (S-P) <br/>+<br/> I23 (X-P)
</td>
</td>
<td>
<td>
-
10/08/2010
+
13/08/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
-
</td><td>
+
</td>
-
<partinfo>pSB1AK3</partinfo>
+
<td>
-
</td><td>
+
<partinfo>pSB1A3</partinfo>
 +
</td>
 +
<td>
Length ok.
Length ok.
</td>  
</td>  
Line 915: Line 920:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I24</font>
+
<font class="BBok">I30</font>
<br/>
<br/>
-
(it was I24-1)
+
(it was ??)
</td>
</td>
<td>
<td>
-
<partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I22 (X-P)
+
<partinfo>BBa_T9002</partinfo> (S-P) <br/>+<br/> I24 (X-P)
</td>
</td>
<td>
<td>
Line 928: Line 933:
</td>
</td>
<td>
<td>
-
<partinfo>pSB1AK3</partinfo>
+
<partinfo>pSB1A3</partinfo>
</td>
</td>
<td>
<td>
Line 942: Line 947:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">I26</font>
+
<font class="BBok">I31</font>
<br/>
<br/>
-
(it was I26-2)
+
(it was ??)
</td>
</td>
<td>
<td>
-
<partinfo>BBa_K105012</partinfo> (S-P) <br/>+<br/> I21-B (X-P)
+
I20 (E-S) <br/>+<br/> <partinfo>BBa_K105012</partinfo> (E-X)
</td>
</td>
<td>
<td>
-
13/08/2010
+
 
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
Line 958: Line 963:
</td>
</td>
<td>
<td>
-
Length ok.
+
 
</td>  
</td>  
<td>
<td>
-
612 bp
+
614 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I32</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I20 (E-S) <br/>+<br/> I21 (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
614 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
<td>
 +
<font class="BBok">I33</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I20 (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1437 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I34</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I21 (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1437 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I35</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I21 (E-S) <br/>+<br/> <partinfo>BBa_K105012</partinfo> (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
614 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I36</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I21 (E-S) <br/>+<br/> I21 (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1158 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I37</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
INTEIN (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1366 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
<td>
 +
<font class="BBok">I38</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
<partinfo>BBa_F2620</partinfo> (E-S) <br/>+<br/> <partinfo>BBa_B0034</partinfo> (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1079 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I39</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J13002</partinfo> (S-P) <br/>+<br/> I20 (X-P)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
652 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I40</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I26 (E-S) <br/>+<br/> INTEIN (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
pMA (commercial vector, HC Amp)
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1125 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 
 +
 
 +
<td>
 +
<font class="BBok">I41</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I26 (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1471 bp
</td>
</td>
<td>Ligations 2010</td>
<td>Ligations 2010</td>
Line 969: Line 1,233:
<tr>
<tr>
<td>
<td>
-
<font class="BBok">INTEIN<br/>MR.G</font>
+
<font class="BBok">INTEIN</font>
</td>
</td>
<td>
<td>
-
Intein
+
Intein (Silver format)
</td>
</td>
<td>
<td>
Line 1,260: Line 1,524:
</tr>
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I27</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I23 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
??
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok
 +
</td>
 +
<td>
 +
725 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I28</font>
 +
<br/>
 +
(it was ??)
 +
</td>
 +
<td>
 +
I24 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
??
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok
 +
</td>
 +
<td>
 +
886 bp
 +
</td>
 +
<td>Ligations 2010</td>
 +
</tr>

Revision as of 09:38, 20 August 2010
Home
The Project
Materials & Methods
Parts
The Team
Notebook
Biological Safety
Pavia
Gallery
Sponsors

FREEZER MANAGEMENT


</tr>

</tr>


</tr>


</tr>


</tr>


</tr>

</tr>


</tr>


</tr>


</tr>


Biobrick name Description Creation date Strain Vector Quality control Length Store

<partinfo>BBa_E2050</partinfo>

mOrange

08/06/2010

E. coli DH5-alpha

<partinfo>pSB2K3</partinfo>

Seq ok (MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>

744 bpBioBricks 2010

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

253 bpBioBricks 2010

<partinfo>BBa_K165037</partinfo>

tef2 promoter

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (MIT)

403 bpBioBricks 2010

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A1</partinfo>

Seq ok(MIT 2009); length ok.

769 bpBioBricks 2010

<partinfo>BBa_J61001</partinfo>

R6K origin

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (MIT)

406 bpBioBricks 2010

<partinfo>BBa_K081008</partinfo>

RBS-luxI

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (MIT); length ok.

664 bpBioBricks 2010

<partinfo>BBa_K125500</partinfo>

GFP fusion brick (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

718 bpBioBricks 2010

<partinfo>BBa_K208001</partinfo>
(stab-1)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

Seq ok (BMR); length ok.

579 bpBioBricks 2010

<partinfo>BBa_K208001</partinfo>
(stab-2)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

Seq ok (BMR); length ok.

579 bpBioBricks 2010

<partinfo>BBa_J72007</partinfo>

BamHI methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here

BioBricks 2010

<partinfo>BBa_J72008</partinfo>

phi80 integration helper plasmid pInt80-649

01/07/2010

E. coli ???

Plasmid (Amp 50 ug/ml resistance)

Length ok

6335 bpBioBricks 2010

<partinfo>BBa_J72013</partinfo>

BglII methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here

BioBricks 2010

<partinfo>BBa_K105012</partinfo>

10 aa flexible protein domain linker

06/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

-

30 bp BioBricks 2010

<partinfo>BBa_J13002</partinfo>

p(tetR)+RBS34

16/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

-

74 bp BioBricks 2010

<partinfo>BBa_I13522</partinfo>

Untagged GFP behind a constitutive promoter

16/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

-

937 bp BioBricks 2010
Ligation name Description Creation date Strain Vector Quality control Length Store

I0
(it was I0-2)

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); length ok.

853 bpLigations 2010

I1
(it was I1-2)

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok.

1029 bp Ligations 2010

I2
(it was I2-1)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); Cm resistance ok; length ok.

906 bpLigations 2010

I3
(it was I3-1)

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.

801 bpLigations 2010

I4
(it was I4-2)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); length ok.

1439 bp Ligations 2010

I5
(it was I5-1)

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR); Cm resistance ok; length ok.

1320 bpLigations 2010

I6
(it was I6-2)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok.

2754 bp Ligations 2010

I7
(it was I7-3)

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Not sequenced, length ok.

2795 bp Ligations 2010

I8
(it was I8-5)

<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Not sequenced, length ok.

2795 bp Ligations 2010

I9
(it was I9-1)

<partinfo>BBa_J23111</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Not sequenced, length ok.

2795 bp Ligations 2010

I10
(it was I10-1)

<partinfo>BBa_J23116</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Not sequenced, length ok.

2795 bp Ligations 2010

I12
(it was I12-2)

<partinfo>BBa_J23105</partinfo> (S-P)
+
I6 (X-P)

16/07/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

2795 bp Ligations 2010

I14
(it was I14-1)

<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp Ligations 2010

RING

I5 (X-S):
Cm resistance (<partinfo>BBa_P1004</partinfo>) + TT (<partinfo>BBa_B0015</partinfo>) + R6K ori (<partinfo>BBa_J61001</partinfo>)

16/07/2010

E. coli DH5-alpha

Plasmid (Cm resistance, conditional thermosensitive ORI; works in PIR strains)

Tested here.

Ligations 2010

I15
(it was I15-1)

<partinfo>BBa_J23110</partinfo> (S-P)
+
I3 (X-P)

23/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

842 bp Ligations 2010

I16
(it was I16-1)

<partinfo>BBa_J23116</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp Ligations 2010

I17
(it was I17-1)

<partinfo>BBa_J23101</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp Ligations 2010

I18
(it was I18-1)

<partinfo>BBa_J23105</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp Ligations 2010

I19
(it was I19-1)

<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp Ligations 2010

I20
(it was I20M-1)

Pha-10S-1 (X-S) (infos here)
+
<partinfo>pSB1AK3</partinfo> (X-S)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

576 bp

Ligations 2010

I21
(it was I21-4)

Pha-SS-1 (X-S) (infos here)
+
<partinfo>pSB1AK3</partinfo> (X-S)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Length ok.

576 bp

Ligations 2010

I22
(it was I22-1)

<partinfo>BBa_K081009</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

825 bp

Ligations 2010

I23
(it was I23-1)

<partinfo>BBa_R0062</partinfo> (S-P)
+
I3 (X-P)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

725 bp

Ligations 2010

I24
(it was I24-1)

<partinfo>BBa_R0062</partinfo> (S-P)
+
I22 (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

886 bp

Ligations 2010

I26
(it was I26-2)

<partinfo>BBa_K105012</partinfo> (S-P)
+
I21-B (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

612 bp

Ligations 2010

I29
(it was ??)

<partinfo>BBa_T9002</partinfo> (S-P)
+
I23 (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Length ok.

725 bp

Ligations 2010

I30
(it was ??)

<partinfo>BBa_T9002</partinfo> (S-P)
+
I24 (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Length ok.

886 bp

Ligations 2010

I31
(it was ??)

I20 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

614 bp

Ligations 2010

I32
(it was ??)

I20 (E-S)
+
I21 (E-X)

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

614 bp

Ligations 2010

I33
(it was ??)

I20 (E-S)
+
I0 (E-X)

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

1437 bp

Ligations 2010

I34
(it was ??)

I21 (E-S)
+
I0 (E-X)

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

1437 bp

Ligations 2010

I35
(it was ??)

I21 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

614 bp

Ligations 2010

I36
(it was ??)

I21 (E-S)
+
I21 (E-X)

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

1158 bp

Ligations 2010

I37
(it was ??)

INTEIN (E-S)
+
I0 (E-X)

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

1366 bp

Ligations 2010

I38
(it was ??)

<partinfo>BBa_F2620</partinfo> (E-S)
+
<partinfo>BBa_B0034</partinfo> (E-X)

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

1079 bp

Ligations 2010

I39
(it was ??)

<partinfo>BBa_J13002</partinfo> (S-P)
+
I20 (X-P)

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

652 bp

Ligations 2010

I40
(it was ??)

I26 (E-S)
+
INTEIN (E-X)

E. coli DH5-alpha

pMA (commercial vector, HC Amp)

1125 bp

Ligations 2010

I41
(it was ??)

I26 (E-S)
+
I0 (E-X)

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

1471 bp

Ligations 2010

INTEIN

Intein (Silver format)

13/08/2010

E. coli TOP10

pMA
(HC Amp resistance)

Mr.Gene DNA synthesis

507 bp

Ligations 2010

CRIM
MR.G

CREAM fragment

13/08/2010

E. coli TOP10

pMK-RQ
(HC Kan resistance)

Mr.Gene DNA synthesis

829 bp

Ligations 2010

I7-4C5
(it was I7-4C5-2)

I7 (E-P)
+
<partinfo>pSB4C5</partinfo>(E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

2795 bp Ligations 2010

I8-4C5
(it was I8-4C5-2)

I8 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

2795 bp Ligations 2010

I10-4C5
(it was I10-4C5-1)

I10 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

2795 bp

Ligations 2010

I12-4C5
(it was I12-4C5-1)

I12 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

2795 bp

Ligations 2010

I14-4C5
(it was I14-4C5-2)

I14 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

23/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

842 bp

Ligations 2010

I15-4C5
(it was I15-4C5-2)

I15 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

Ligations 2010

I16-4C5
(it was I16-4C5-1)

I16 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

23/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

842 bp

Ligations 2010

I17-4C5
(it was I17-4C5-1)

I17 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

842 bp

Ligations 2010

I18-4C5
(it was I18-4C5-1)

I18 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

842 bp

Ligations 2010

I19-4C5
(it was I19-4C5-1)

I19 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

842 bp

Ligations 2010

I27
(it was ??)

I23 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

??

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

725 bp

Ligations 2010

I28
(it was ??)

I24 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

??

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok

886 bp

Ligations 2010
Strain name Description Arrival date Infos Resistance Quality control Notes Store

MG1655(Seq)
(MG)

E. coli wild type strain fully sequenced

28/06/2010

link

- Genome fully sequenced Strains 2010

MC1061
(MC)

E. coli strain

28/06/2010

link

- Strain used by JC Anderson et al. for integration Strains 2010

BW25141
(BW1)

E. coli pir+ strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K LC pir Strains 2010

BW25142
(BW2)

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW23473

E. coli pir+ strain

19/07/2010

link

- LC pir Strains 2010

BW23474

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW5328/pAH123

E. coli strain

28/06/2010

link

Amp 50 ug/ml Plasmid length ok (after digestion with SpeI).

Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C

Strains 2010

BT340

E. coli strain

28/06/2010

link

Amp 100 ug/ml and Cm 34 ug/ml Plasmid relative molecular weight ok (after gel run of the undigested plasmid)

Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C

Strains 2010

DSM15372

E. coli DMS15372 strain

08/07/2010

link

Amp 100 ug/ml Length ok (after digestion with EcoRI)

Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon)

Strains 2010
Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Freezer"