Team:UNIPV-Pavia/Freezer

From 2010.igem.org

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<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
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<table border="0" align="center" width="100%" class="freezer"><tr><td align="justify" valign="top" style="padding:20px">
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<html><p align="center"><font size="4"><b>FREEZER MANAGEMENT</b></font></p></html><hr><br>
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<html><p align="center"><font size="4" color=#FFFFFF><b>FREEZER MANAGEMENT</b></font></p></html><hr><br>
<table class="cont" border=0>
<table class="cont" border=0>
<tr><th>Biobrick or<br>ligation name</th><th>Description</th><th>Creation date</th><th>Strain</th><th>Vector</th><th>Quality control</th><th>Length</th><th>Store</th> </tr>
<tr><th>Biobrick or<br>ligation name</th><th>Description</th><th>Creation date</th><th>Strain</th><th>Vector</th><th>Quality control</th><th>Length</th><th>Store</th> </tr>

Revision as of 14:36, 11 July 2010
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FREEZER MANAGEMENT



Biobrick or
ligation name		DescriptionCreation dateStrainVectorQuality controlLengthStore

<partinfo>BBa_E2050</partinfo>

mOrange

08/06/2010

E. coli DH5-alpha

<partinfo>pSB2K3</partinfo>

Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>

744 bpBioBricks 2010

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

253 bpBioBricks 2010

<partinfo>BBa_K165037</partinfo>

tef2 promoter

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(MIT)

403 bpBioBricks 2010

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A1</partinfo>

Seq ok(MIT 2009); length ok

769 bpBioBricks 2010

<partinfo>BBa_J61001</partinfo>

R6K origin

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(MIT)

406 bpBioBricks 2010

<partinfo>BBa_K081008</partinfo>

RBS-luxI

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(MIT); length ok

664 bpBioBricks 2010

<partinfo>BBa_K125500</partinfo>

GFP fusion brick (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

718 bpBioBricks 2010

I0-1

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Wrong clone

Ligations 2010

I0
(it was I0-2)

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); length ok

853 bpLigations 2010

I1-1
(not used)

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

not sequenced, length ok

Ligations 2010

I1
(it was I1-2)

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok

1029 bpLigations 2010

I2
(it was I2-1)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); Cm resistance ok; length ok.

906 bpLigations 2010

I2-2
(not used)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

not sequenced; Cm resistance ok

Ligations 2010

I3
(it was I3-1)

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok

801 bpLigations 2010

I3-2

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Wrong clone

Ligations 2010

I4-1
(not used)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

not sequenced; length ok

Ligations 2010

I4
(it was I4-2)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok(BMR); length ok

1439 bpLigations 2010

I5
(it was I5-1)

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok(BMR); Cm resistance ok; length ok

1320 bpLigations 2010

I6
(it was I6-2)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok

2754 bpLigations 2010

I6-3
(not used)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

not sequenced

Ligations 2010

<partinfo>BBa_K208001</partinfo> (stab-1)

 phasin phaP

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

length ok

579 bpBioBricks 2010

<partinfo>BBa_K208001</partinfo> (stab-2)

 phasin phaP

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

length ok

579 bpBioBricks 2010

I7-1

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Wrong clone

Ligations 2010

I7-2

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Wrong clone

Ligations 2010

I7
(it was I7-3)

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010

I7-5

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010

I8-1

 <partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Wrong clone

Ligations 2010

I8-2

 <partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Wrong clone

Ligations 2010

I8-4

 <partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010

I8
(it was I8-5)

<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010

I9
(it was I9-1)

<partinfo>BBa_J23111</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010

I9-2

 <partinfo>BBa_J23111</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010

I10
(it was I10-1)

<partinfo>BBa_J23116</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010

I10-2

 <partinfo>BBa_J23116</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>

Not sequenced, length ok

Ligations 2010
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