Team:UNIPV-Pavia/Calendar/July/settimana3

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JULY: WEEK 3



July, 12th

Gel run/cut of phasins amplified via PCR

July, 13th

July, 14th

Quality control for HELPER and CRIM plasmids

July, 15th

File:UNIPV10 1 QC CRIM pBHR68.jpg
Quality control for pBHR68 and CRIM plasmids

July, 16th

Screening ligations I11-I16
Screening ligations I17-I19, I7/I8/I104C5

Since it's impossible to isolate the CRIM plasmid <partinfo>BBa_J72007</partinfo> we created a pseudo CRIM plasmid to check that not pir strains aren't able to propagate it. We called it "THE RING" (RING) and we built it from our I5 (Cm resistance - <partinfo>BBa_P1004</partinfo> - + TT - <partinfo>BBa_B0015</partinfo> - + R6K ori - <partinfo>BBa_J61001</partinfo>) in <partinfo>pSB1A2</partinfo>:

  • Digestion of I5 XbaI-PstI
  • Gel extraction
  • Quantification with Nanodrop (15ng/ul)
  • Ligation of X and P sites each other (used 2ul of DNA)


Calendar

July

week 1

week 2

week 3

week 4

week 5