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Team:UNIPV-Pavia/Calendar/July/settimana2
From 2010.igem.org
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<html><p align="center"><font size="4"><b>JULY: WEEK 2</b></font></p></html><hr><br> | <html><p align="center"><font size="4"><b>JULY: WEEK 2</b></font></p></html><hr><br> | ||
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==July, 5th== | ==July, 5th== | ||
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*LB (250ml) | *LB (250ml) | ||
*LB+Cm 12.5 (250ml) | *LB+Cm 12.5 (250ml) | ||
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| + | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==July, 6th== | ==July, 6th== | ||
| - | We decided to perform a | + | We decided to perform a TECAN test to evaluate the strength of some promoters belonging to the Anderson Promoters Collection. Inoculum from glycerol stocks (8ul in 5ml LB) for: |
*<partinfo>BBa_J23101</partinfo> | *<partinfo>BBa_J23101</partinfo> | ||
*<partinfo>BBa_J23110</partinfo> | *<partinfo>BBa_J23110</partinfo> | ||
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TECAN experiment started at 10:20am. | TECAN experiment started at 10:20am. | ||
| - | In the morning one single colony was | + | In the morning one single colony was picked for BW53474 E. coli strain and glycerol stock was prepared after about 7 hours (37°C 220rpm) |
Inoculum of: | Inoculum of: | ||
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*BW25141 (from glycerol stock) | *BW25141 (from glycerol stock) | ||
*BW25142 (from glycerol stock) | *BW25142 (from glycerol stock) | ||
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| + | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==July, 7th== | ==July, 7th== | ||
| - | Competent cells | + | Competent cells preparation for: |
*MG1655 | *MG1655 | ||
*BW53474 | *BW53474 | ||
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All parts were correct!! :) | All parts were correct!! :) | ||
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| + | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==July, 8th== | ==July, 8th== | ||
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Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C. | Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C. | ||
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| + | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==July, 9th== | ==July, 9th== | ||
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| DH5alpha || 7475<br/>(only 100ul of cells plated) || ~ 10^6<br/>(very difficult to count correctly,<br/>previous tests showed an efficiency of ~10^8) | | DH5alpha || 7475<br/>(only 100ul of cells plated) || ~ 10^6<br/>(very difficult to count correctly,<br/>previous tests showed an efficiency of ~10^8) | ||
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| + | <div align="right"><small>[[#indice|^top]]</small></div> | ||
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<!-- table previous next week --> | <!-- table previous next week --> | ||
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Latest revision as of 16:53, 24 October 2010
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