Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/08

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===Experiment:digestion of bcsA,B,C(from''E.coli'')===
===Experiment:digestion of bcsA,B,C(from''E.coli'')===
'''member'''<br />
'''member'''<br />
-
bambi73 and NEX
+
bambi75 and NEX
'''material'''<br />
'''material'''<br />

Revision as of 05:10, 8 September 2010

2010/9/8 Monday (NEX)

Experiment:digestion of bcsA,B,C(fromE.coli)

member
bambi75 and NEX

material

  • bcsA,B,C(from E.coli)
  • 10×Mbuffer
  • BSA
  • XbaI
  • SpeI

procedure

  1. add "material" to PCR tubes(show below)
  2. incubation at (37℃,7h)
composition
bcsAbcsBbcsC
solution of bcs(μl) 606060
10×Mbuffer(μl) 6 6 6
BSA(μl) 2 2
XbaI(μl) 1.21.2
SpeI(ul) 1.21.2

Experiment:Make Agarose Gels Electro-phoresis

Member
Same above

Materials

  • 1×TaE 200ml
  • Agarose 2g
  • Ethidium bromide　10µl

Procedure

  1. Add and mixture above materials in an Erlenmeyer flask
  2. Warm up them in a Microwave oven
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