Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/08
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(Difference between revisions)
(New page: ==2010/9/8 Monday (NEX)== ===Experiment:digestion of bcsA,B,C,D(''A.xylinus'')=== '''member'''<br /> bambi73 and NEX '''material'''<br /> *bcsA,B,C(from ''E.coli'') *10×Mbuffer *BSA ...) |
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! XbaI(μl) | ! XbaI(μl) | ||
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! SpeI(ul) | ! SpeI(ul) | ||
- | | | + | | 1.2||1.2|| || |
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===Experiment:Make Agarose Gels Electro-phoresis=== | ===Experiment:Make Agarose Gels Electro-phoresis=== |
Revision as of 05:06, 8 September 2010
Experiment:digestion of bcsA,B,C,D(A.xylinus)
member
bambi73 and NEX
material
- bcsA,B,C(from E.coli)
- 10×Mbuffer
- BSA
- XbaI
- SpeI
procedure
- add "material" to PCR tubes(show below)
- incubation at (37℃,7h)
bcsA | bcsB | bcsC | ||
---|---|---|---|---|
solution of bcs(μl) | 60 | 60 | 60 | |
10×Mbuffer(μl) | 6 | 6 | 6 | |
BSA(μl) | 2 | 2 | ||
XbaI(μl) | 1.2 | 1.2 | ||
SpeI(ul) | 1.2 | 1.2 |
Experiment:Make Agarose Gels Electro-phoresis
Member
Same above
Materials
- 1×TaE 200ml
- Agarose 2g
- Ethidium bromide 10µl
Procedure
- Add and mixture above materials in an Erlenmeyer flask
- Warm up them in a Microwave oven