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From 2010.igem.org

Contents 1 2010/8/30 Monday (Naoto) 1.1 Experiment:electrophoresis 1.2 Experiment:separation of A.xylinus 1.3 Experiment:direct PCR 1.4 Experiment:purification of agarose gel 1.5 Experiment:electrophoresis 1.6 Experiment:purification of agarose gel

2010/8/30 Monday (Naoto)

Experiment:electrophoresis

member
NEX,bambi75 and watachin

material

• PCR production (bcsC from E.coli)
• 1×TAE buffer
• agarose gel

procedure
follow to protocol4

result
bands of bcsC were appeared

Experiment:separation of A.xylinus

member
naoto

material

• A.xylinus in open wet ware media(8/25 made)

procedure

1. take 0.5ml of A.xylinus in open wet ware media to tubes
2. centrifuge 15000rpm/5min

Experiment:direct PCR

member
naoto

material

• A.xylinus separated above experiment

procedure
follow to protocol3

Experiment:purification of agarose gel

member
NEX,bambi75 and watachin

material

• bcsC from E.coli in agarose gel
• QIAGEN

procedure
follow to protocol5

Experiment:electrophoresis

member
naoto

material

• PCR production (bcsA from A.xylinus)
• 1×TAE buffer
• agarose gel

procedure
follow to protocol4

result
a band of bcsA were appeared (below bands appreared to be primer)

Experiment:purification of agarose gel

member
naoto

material

• bcsA from A.xylinus in agarose gel
• QIAGEN

procedure
follow to protocol5