Team:TU Munich/Parts
From 2010.igem.org
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New PartsSingle PartsBBa_K494000
PlasmidsBBa_K494001New, better pSB1A10 We recloned pSB1A10 to improve its features as a measuring plasmid to evaluate terminators in vivo using fluorescent proteins as reporters. RFP which was known to contain an RNase restriction site was exchanged against mCherry which combines good expression yield, short maturation times and an acceptable and well-characterized quantum yield. For easy introduction of the terminator to be evaluated, we ?? ?? ?? BBa_K494002Positive control BBa_K494003With His-Term/Signal BBa_K494004With Trp-Term/Signal
FalsificationpSB1A10 |