Use of provided BioBricks
Making Competent Cells
Transformation home-made competent cells
Transformation commercial competent cells
Transformation efficientcy (CFU/µg)
Birnboim plasmid isolation
Qiagen Mini-prep plasmid isolation
Qiagen Midi-prep plasmid isolation
Freezing of bacterial stocks
Restriction enzyme digestion
Ligation
Agarose gel
PCR
Overlapping PCR
Colony PCR
Qiagen Gel extraction
Precipitation of PCR products
Protein purification
Protein content measurments (Bradford assay)
Emulsifier test
Buffers and stock solutions
Primers
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