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Team:TU Delft/2 July 2010 content
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| - | At the same time Eva is making more [[Team:TU_Delft/protocols/colony_PCR| | + | At the same time Eva is making more [[Team:TU_Delft/protocols/colony_PCR|competent cells]]. We go through our competent cells so quickly. |
To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature. | To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature. | ||
Revision as of 19:02, 12 July 2010
Lab work
Today we performed Colony PCR with the transformants of the BioBricks to check if everything is what is says it is.
Results of the colony PCR on 1% agarose gel
- Marker 1: EZ load percision
- Marker 2: smartladder
- Sample 1: J61100
- Sample 2: J61101
- Sample 3: J61107
- Sample 4: J61117
- Sample 5: J61127
- Sample 6: B0034
- Sample 7: J23109
- Sample 8: B0015
- Sample 9: E0422
- Sample 10: E0240
All the BioBricks have the correct size.
At the same time Eva is making more competent cells. We go through our competent cells so quickly. To test the efficiency of the competent cells, 100 ng standard pUC19 vector was transformed and plated on LB agar containing 100 μg/mL Ampicillin. The plates were incubated on room temperature.
World cup
We watched the World Cup game (the Netherlands - Brazil) in our iGEM room. Care was taken for many tasty snacks. Cake, chips and drinks Mmmmm.
