Team:Stockholm/26 September 2010
From 2010.igem.org
(Difference between revisions)
JohanNordholm (Talk | contribs)
(New page: {{Stockholm/Top2}} =Johan= ==Colony PCR screen== bFGF-his and his-bFGF in pMA (vector with histag) 0,5 µl pol 0,5 µl dNTP 5 µl 10x buffer 1,5 µl pMA for primer 1,5 µl pMA rev ...)
(New page: {{Stockholm/Top2}} =Johan= ==Colony PCR screen== bFGF-his and his-bFGF in pMA (vector with histag) 0,5 µl pol 0,5 µl dNTP 5 µl 10x buffer 1,5 µl pMA for primer 1,5 µl pMA rev ...)
Latest revision as of 22:03, 27 October 2010
Johan
Colony PCR screen
bFGF-his and his-bFGF in pMA (vector with histag)
0,5 µl pol
0,5 µl dNTP
5 µl 10x buffer
1,5 µl pMA for primer
1,5 µl pMA rev primer
16 µl H2O
10 colonies from both plates
Gel
Results: All colonies correct! Expected as both insert and vector had been gel purified.