Team:Stanford/NotebookPage/6 July 2010

From 2010.igem.org

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{{Template: Stanford Notebook Calendar}}
{{Template: Stanford Notebook Calendar}}
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[https://docs.google.com/document/edit?id=1xnkLfvrwGJuU1Z_9-y3R0topqahNiJUQpdpNOWlK9Ig&hl=en&authkey=CNnS3IEB Agenda]
Alex's References
Alex's References
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#[http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis]
#[http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis]
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<div style=text-align:left justify">
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=Greg's Lab Notebook=
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*During weekly meeting got up to speed with what the team’s been doing while I was away
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*Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:
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{| class="wikitable" style="text-align: center;"
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|-
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! Function !! Part # !! Distribution Location !! Resistance
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|-
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| ribosome binding site || B0032 || P1 2I || Amp
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|-
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| forward double terminator || B0015 || P1 23L || Amp + Kan
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|-
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| bidirectional double terminator || B0014 || P2 24C || Amp + Kan
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|-
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| medium constitutive promoter || J23107 || P1 20A || Amp
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|-
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| strong constitutive promoter || J23100 || P1 18C || Amp
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|-
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| plasmid backbone || pSB1K3 || P1 5A || Kan
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|}

Latest revision as of 20:27, 12 July 2010

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Home Project Protocols Parts Notebook Team Archives

March
MTWTFSS
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_March_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_March_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_March_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_March_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_March_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_March_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_March_2010&action=edit 7]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_March_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_March_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_March_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_March_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_March_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_March_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_March_2010&action=edit 14]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_March_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_March_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_March_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_March_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_March_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_March_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_March_2010&action=edit 21]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_March_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_March_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_March_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_March_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_March_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_March_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_March_2010&action=edit 28]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_March_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_March_2010&action=edit 30] [http://2010.igem.org/Team:Stanford/NotebookPage/31_March_2010 31]
April
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_April_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_April_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_April_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_April_2010&action=edit 4]
[http://2010.igem.org/Team:Stanford/NotebookPage/5_April_2010 5] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_April_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_April_2010&action=edit 7] [http://2010.igem.org/Team:Stanford/NotebookPage/8_April_2010 8] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_April_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_April_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_April_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_April_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_April_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_April_2010&action=edit 14] [http://2010.igem.org/Team:Stanford/NotebookPage/15_April_2010 15] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_April_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_April_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_April_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_April_2010&action=edit 19] [http://2010.igem.org/Team:Stanford/NotebookPage/20_April_2010 20] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_April_2010&action=edit 21] [http://2010.igem.org/Team:Stanford/NotebookPage/22_April_2010 22] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_April_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_April_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_April_2010&action=edit 25]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_April_2010&action=edit 26] [http://2010.igem.org/Team:Stanford/NotebookPage/27_April_2010 27] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_April_2010&action=edit 28] [http://2010.igem.org/Team:Stanford/NotebookPage/29_April_2010 29] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_April_2010&action=edit 30]
May
MTWTFSS
          [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_May_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_May_2010&action=edit 2]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_May_2010&action=edit 3] [http://2010.igem.org/Team:Stanford/NotebookPage/4_May_2010 4] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_May_2010&action=edit 5] [http://2010.igem.org/Team:Stanford/NotebookPage/6_May_2010 6] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_May_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_May_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_May_2010&action=edit 9]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_May_2010&action=edit 10] [http://2010.igem.org/Team:Stanford/NotebookPage/11_May_2010 11] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_May_2010&action=edit 12] [http://2010.igem.org/Team:Stanford/NotebookPage/13_May_2010 13] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_May_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_May_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_May_2010&action=edit 16]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_May_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_May_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_May_2010&action=edit 19] [http://2010.igem.org/Team:Stanford/NotebookPage/20_May_2010 20] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_May_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_May_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_May_2010&action=edit 23]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_May_2010&action=edit 24] [http://2010.igem.org/Team:Stanford/NotebookPage/25_May_2010 25] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_May_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_May_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_May_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_May_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_May_2010&action=edit 30]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/31_May_2010&action=edit 31]
June
MTWTFSS
  [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_June_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_June_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_June_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_June_2010&action=edit 6]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_June_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_June_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_June_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_June_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_June_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_June_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_June_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_June_2010&action=edit 27]
[http://2010.igem.org/Team:Stanford/NotebookPage/28_June_2010 28] [http://2010.igem.org/Team:Stanford/NotebookPage/29_June_2010 29] [http://2010.igem.org/Team:Stanford/NotebookPage/30_June_2010 30]
July
MTWTFSS
      [http://2010.igem.org/Team:Stanford/NotebookPage/1_July_2010 1] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_July_2010&action=edit 5] [http://2010.igem.org/Team:Stanford/NotebookPage/6_July_2010 6] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_July_2010&action=edit 7] [http://2010.igem.org/Team:Stanford/NotebookPage/8_July_2010 8] [http://2010.igem.org/Team:Stanford/NotebookPage/9_July_2010 9] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_July_2010&action=edit 11]
[http://2010.igem.org/Team:Stanford/NotebookPage/12_July_2010 12] [http://2010.igem.org/Team:Stanford/NotebookPage/13_July_2010 13] [http://2010.igem.org/Team:Stanford/NotebookPage/14_July_2010 14] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_July_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_July_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_July_2010&action=edit 18]
[http://2010.igem.org/Team:Stanford/NotebookPage/19_July_2010 19] [http://2010.igem.org/Team:Stanford/NotebookPage/20_July_2010 20] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_July_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_July_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_July_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_July_2010&action=edit 25]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_July_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_July_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_July_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_July_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_July_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_August_2010&action=edit 1]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_August_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_August_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_August_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_August_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_August_2010&action=edit 8]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_August_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_August_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_August_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_August_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_August_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_August_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_August_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_August_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_August_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_August_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_August_2010&action=edit 22]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_August_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_August_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_August_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_August_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_August_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_August_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/31_August_2010&action=edit 31]
September
MTWTFSS
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[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_September_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_September_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_September_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_September_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_September_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_September_2010&action=edit 12]
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[http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_September_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_September_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_September_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_September_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_September_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_September_2010&action=edit 26]
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October
MTWTFSS
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Agenda

Alex's References

  1. [http://www.nature.com/ja/journal/v59/n2/abs/ja200618a.html Self-activation of Serine/Threonine Kinase AfsK on Autophosphorylation at Threonine-168]
  2. [http://www.ncbi.nlm.nih.gov/pubmed/11952895 afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2).]

Chris's references

  1. [http://www3.interscience.wiley.com/journal/118634717/abstract?CRETRY=1&SRETRY=0 EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine/threonine kinases and phosphatase in Mycobacterium tuberculosis]
  2. [http://www.ncbi.nlm.nih.gov/pubmed/18424526 The critical role of embC in Mycobacterium tuberculosis.]
  3. [http://jb.asm.org/cgi/content/abstract/188/8/2936 Transcriptional Control of the Mycobacterial embCAB Operon by PknH through a Regulatory Protein, EmbR, In Vivo]
  4. [http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis]

Greg's Lab Notebook

  • During weekly meeting got up to speed with what the team’s been doing while I was away
  • Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:


Function Part # Distribution Location Resistance
ribosome binding site B0032 P1 2I Amp
forward double terminator B0015 P1 23L Amp + Kan
bidirectional double terminator B0014 P2 24C Amp + Kan
medium constitutive promoter J23107 P1 20A Amp
strong constitutive promoter J23100 P1 18C Amp
plasmid backbone pSB1K3 P1 5A Kan
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