Team:SDU-Denmark/project-p

From 2010.igem.org

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(Computerized analysis of the bacterial motility with the THOR prototype by Unisensor A/S and the Unify software)
(Computerized analysis of the bacterial motility with the THOR prototype by Unisensor A/S and the Unify software)
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The bacteria that are not moving in the video are assumed to be sticking to the glass or coverslip.<br>
The bacteria that are not moving in the video are assumed to be sticking to the glass or coverslip.<br>
From these videos we extracted data through Unisensor's in-house programmed software Unify and it's tracking extension. For each frame the bacteria were identified and their location compared to that of the frame before it and by thta, tracking the bacteria over time. The resulting data that was output for each frame was: Location of bacteria given as coordinates (X,Y), bacterial orientation in angle ranging from -180° to 180° and length of the path was tracked in pixels. An example of the unify sofware analysing the paths of the bacteria and at the same creating a video of the individual tracks:<br>
From these videos we extracted data through Unisensor's in-house programmed software Unify and it's tracking extension. For each frame the bacteria were identified and their location compared to that of the frame before it and by thta, tracking the bacteria over time. The resulting data that was output for each frame was: Location of bacteria given as coordinates (X,Y), bacterial orientation in angle ranging from -180° to 180° and length of the path was tracked in pixels. An example of the unify sofware analysing the paths of the bacteria and at the same creating a video of the individual tracks:<br>
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Here is an example of a .txt file containing the results: [http://2010.igem.org/wiki/images/6/6b/TrackResult_iGEM-WTred1.txt Tracks of wildtype bacteria exposed to red light]. If interested, please request the whole dataset from us, which will be sent out as a 450mb zip file.<br>
Here is an example of a .txt file containing the results: [http://2010.igem.org/wiki/images/6/6b/TrackResult_iGEM-WTred1.txt Tracks of wildtype bacteria exposed to red light]. If interested, please request the whole dataset from us, which will be sent out as a 450mb zip file.<br>
From this file, a trackplot was generated, showing each bacteria's path over the recorded time:<br>
From this file, a trackplot was generated, showing each bacteria's path over the recorded time:<br>

Revision as of 19:01, 27 October 2010