Team:SDU-Denmark/project-p

From 2010.igem.org

(Difference between revisions)
(Phase contrast of ON cultures)
(Computerized analysis of the bacterial motility with the THOR prototype by Unisensor A/S and the Unify software)
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Contrary to our expectations the plot over mean velocity/frame did not show a noticeable difference between the four samples. The photosensor had about the same velocity/frame in blue and red light and there was no clear difference either when compared to the wildtype. The reason for this could be that we were were not effective enough at excluding non-informative paths from the data file (bacteria that are trapped in a circular motion, bacteria only exhibiting brownian motion) and / or that the environment the experiments were done in, was not optimal in the meaning of that the photosensor was exposed to light, before the measurements.<br>
Contrary to our expectations the plot over mean velocity/frame did not show a noticeable difference between the four samples. The photosensor had about the same velocity/frame in blue and red light and there was no clear difference either when compared to the wildtype. The reason for this could be that we were were not effective enough at excluding non-informative paths from the data file (bacteria that are trapped in a circular motion, bacteria only exhibiting brownian motion) and / or that the environment the experiments were done in, was not optimal in the meaning of that the photosensor was exposed to light, before the measurements.<br>
Since the results from the experiment with the red and blue light were inconclusive, we went on to analyse the data from the experiment, where we set up a light gradient in the area that was observed through the microscope. We recorded this for 40 seconds with a framerate of 4 frames/second, waited for 100 seconds and recorded again for 40 seconds. We did 10 iterations of this, which would give us a long-term overview of migration (if there is any) along the light gradient. Afterwards we repeated the same experiment without stimulating the bacteria through a light gradient. A track diagram was created, which should show us if the tracked microbes would show any preference in one direction. In the circular diagram each abcteria starts at point zero and then moves is plotted by its angle and distance traveled. This will give us an idea if there is any tendency to either move towards or away from the light:
Since the results from the experiment with the red and blue light were inconclusive, we went on to analyse the data from the experiment, where we set up a light gradient in the area that was observed through the microscope. We recorded this for 40 seconds with a framerate of 4 frames/second, waited for 100 seconds and recorded again for 40 seconds. We did 10 iterations of this, which would give us a long-term overview of migration (if there is any) along the light gradient. Afterwards we repeated the same experiment without stimulating the bacteria through a light gradient. A track diagram was created, which should show us if the tracked microbes would show any preference in one direction. In the circular diagram each abcteria starts at point zero and then moves is plotted by its angle and distance traveled. This will give us an idea if there is any tendency to either move towards or away from the light:
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[[Image:TrackDiagramGradient.png]]
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[[Image:TrackDiagramGradient.png|thumb|640px|center|Track diagram of phototaxic bacteria swimming, when exposed to a gradient. ]]

Revision as of 18:30, 27 October 2010