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Team:Queens-Canada/30 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: <h2>July 30, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Thai '''pRab-7'''::1A3, '''pMec-7'''::1A3, '''unc-54 3'UTR'''::1A3, '''pSt...) |
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Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 110 V. | Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 110 V. | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | Practiced injections with Sur-5::GFP marker. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Revision as of 01:56, 28 October 2010
July 30, 2010
Miniprep
Thai
pRab-7::1A3, pMec-7::1A3, unc-54 3'UTR::1A3, pStr-1::1A3
Minprep
Hao and Steve
pSB1A3, pSB1T3, pSB1K3, pSB1C3, pStr-1::1A2, pSip-1::1A3, pRab-7::1A3
Gel Electrophoresis
Thai and Yuli
pSB1A3, unc-54 3'UTR::1A3
Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.
Gel Electrophoresis
Thai and Yuli
pSB1T3, pSB1K3, pSB1C3, pSip-1::1A3, pRab-7::1A3, pMec-7::1A3, pStr-1::1A2
Gel Electrophoresis
Hao and Yuli
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 110 V.
Microinjection
Hao
Practiced injections with Sur-5::GFP marker.
