Team:Northwestern/Project/Lac

From 2010.igem.org

(Difference between revisions)
Line 110: Line 110:
<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
-
{|- style="font-size: 87%; background: #AFC7C7; color: #000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
+
{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
-
!align="center"|[[Team:Northwestern|<font color="#FFFFFF">'''Home'''</font>]]
+
!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
-
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#FFFFFF">'''Brainstorm'''</font>]]
+
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
-
!align="center"|[[Team:Northwestern/Team|<font color="#FFFFFF">'''Team'''</font>]]
+
!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
-
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#FFFFFF">'''Acknowledgements'''</font>]]
+
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
-
!align="center"|[[Team:Northwestern/Project|<font color="#2B3856">'''Project'''</font>]]
+
!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
-
!align="center"|[[Team:Northwestern/SideProject|<font color="#FFFFFF">'''Human Practices'''</font>]]
+
!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
-
!align="center"|[[Team:Northwestern/Parts|<font color="#FFFFFF">'''Parts'''</font>]]
+
!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
-
!align="center"|[[Team:Northwestern/Notebook|<font color="#FFFFFF">'''Notebook'''</font>]]
+
!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
-
!align="center"|[[Team:Northwestern/Calendar|<font color="#FFFFFF">'''Calendar'''</font>]]
+
!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
-
!align="center"|[[Team:Northwestern/Protocol|<font color="#FFFFFF">'''Protocol'''</font>]]
+
!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
-
!align="center"|[[Team:Northwestern/Safety|<font color="#FFFFFF">'''Safety'''</font>]]
+
!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
-
!align="center"|[[Team:Northwestern/Links|<font color="#FFFFFF">'''Links'''</font>]]
+
!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
-
!align="center"|[[Team:Northwestern/References|<font color="#FFFFFF">'''References'''</font>]]
+
!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
-
!align="center"|[[Team:Northwestern/Media|<font color="#FFFFFF">'''Media'''</font>]]
+
!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
-
!align="center"|[[Team:Northwestern/Contact|<font color="#FFFFFF">'''Contact'''</font>]]
+
!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
|}
|}

Revision as of 16:27, 23 October 2010


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Modeling Chassis Induction Chitin Apoptosis

For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information.

The induction construct consisted of a constitutive promoter (J23100, J23104, or J23105), a combination part of Ribosome Binding Site, Lac Repressor, double Terminators, and Lac Promoter (Q01121, Q04121), and Ribosome Binding Sequence (B0034, B0031, B0032).

Multiple versions of each part were used to generate a wide range of repressor concentrations, so as to allow for variation between the induction time-delay and degree of Apoptosis protein and Chitin Synthase synthesis.

The effectiveness of these constructs were tested in the modeling section.